Capillarisin Exhibits Anticancer Effects by Inducing Apoptosis, Cell Cycle Arrest and Mitochondrial Membrane Potential Loss in Osteosarcoma Cancer Cells (HOS)

N.-J. Chen; F.-Y. Hao; H. Liu; H. Zhao; J.-M. Li

doi:10.1055/s-0034-1387728

Drug Research, Table of Contents

Drug Res (Stuttg) 2015; 65(08): 422-427
DOI: 10.1055/s-0034-1387728

Original Article

Capillarisin Exhibits Anticancer Effects by Inducing Apoptosis, Cell Cycle Arrest and Mitochondrial Membrane Potential Loss in Osteosarcoma Cancer Cells (HOS)

N.-J. Chen

¹Department of Orthopaedics, Qilu Hospital, Shandong University, Jinan, China

⁴Department of Orthopaedics, Zibo Central Hospital, Zibo, China

,

F.-Y. Hao

²Department of Pathology, The Affiliated Hospital of Medical College Qingdao University, Qingdao, China

,

H. Liu

³Department of Radiology, Zibo Central Hospital, Zibo, China

,

H. Zhao

⁴Department of Orthopaedics, Zibo Central Hospital, Zibo, China

,

J.-M. Li

¹Department of Orthopaedics, Qilu Hospital, Shandong University, Jinan, China

› Author Affiliations

Abstract

The aim of the present study was to assess the anticancer activity of capillarisin against human osteosarcoma (HOS) cancer cells in vitro. Cell viability after capillarisin drug treatment and evaluated by MTT assay. The extent of cell death induced by capillarisin was estimated by using lactate dehydrogenase (LDH) assay. The effect of capillarisin on cell cycle phase distribution and mitochondrial membrane potential (ΛΨm) was demonstrated via flow cytometry using propidium iodide (PI) and rhodamine-123 (Rh-123) DNA-binding fluorescent dyes respectively. Fluorescence microscopy was employed to examine the morphological changes in osteosarcoma cancer cells and presence of apoptotic bodies following capillarisin treatment. The results of this study revealed that capillarisin induced dose-dependent growth inhibition of these cancer cells after 12-h of incubation. Further, capillarisin induced significant release of LDH from these cell cultures and this LDH release was much more noticeable at higher concentrations of capillarisin. Hoechst 33258 staining revealed characteristic morphological features of apoptosis triggered by capillarisin treatment. Cell cycle analysis revealed that capillarisin induced dose-dependent G0/G1-phase cell cycle arrest. Capillarisin also trigerred a progressive and dose-dependent reduction in the mitochondrial membrane potential. In conclusion, capillarisin inhibits cancer cell growth of osteosarcoma cells by inducing apoptosis accompanied with G0/G1-phase cell cycle arrest and loss in mitochondrial membrane potential.

Key words

capillarisin - anticancer - osteosarcoma - flow cytometry - mitochondrial membrane potential

Full Text

References

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