Eur J Pediatr Surg 2015; 25(01): 56-59
DOI: 10.1055/s-0034-1387951
Original Article
Georg Thieme Verlag KG Stuttgart · New York

Raman Spectroscopy in the Diagnosis of Ulcerative Colitis

Michelle Anne Veenstra
1   Department of Pediatric Surgery, Children's Hospital of Michigan, Detroit, Michigan, United States
2   Department of General Surgery, Wayne State University, Detroit, Michigan, United States
,
Olena Palyvoda
3   Department of Electrical and Computer Engineering, Wayne State University, Detroit, Michigan, United States
,
Hazem Alahwal
2   Department of General Surgery, Wayne State University, Detroit, Michigan, United States
,
Marko Jovanovski
2   Department of General Surgery, Wayne State University, Detroit, Michigan, United States
,
Luke Anthony Reisner
3   Department of Electrical and Computer Engineering, Wayne State University, Detroit, Michigan, United States
,
Brady King
1   Department of Pediatric Surgery, Children's Hospital of Michigan, Detroit, Michigan, United States
2   Department of General Surgery, Wayne State University, Detroit, Michigan, United States
,
Janet Poulik
4   Department of Pathology, Children's Hospital of Michigan, Detroit, Michigan, United States
,
Michael D. Klein
1   Department of Pediatric Surgery, Children's Hospital of Michigan, Detroit, Michigan, United States
5   Department of Surgery, Wayne State University School of Medicine, Detroit, Michigan, United States
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Weitere Informationen

Publikationsverlauf

01. Juli 2014

02. Juli 2014

Publikationsdatum:
30. August 2014 (online)

Abstract

Introduction At present, the diagnosis of ulcerative colitis (UC) requires the histologic demonstration of characteristic mucosal inflammatory changes. A rapid and noninvasive diagnosis would be of value, especially if it could be adapted to a simple rectal probe. Raman spectroscopy creates a molecular fingerprint of substances by detecting laser light scattered from asymmetric, vibrating, and chemical bonds. We hypothesize that Raman spectroscopy can distinguish UC from non-UC colon tissue rapidly and accurately.

Materials and Methods Colon tissue specimens were obtained from patients operated at the Children's Hospital of Michigan, United States, including UC colon and non-UC colon. The samples were examined with a Renishaw inVia Raman microscope (Gloucestershire, United Kingdom) with a 785 nm laser. Principal component analysis and discriminant function analysis were used to classify groups. Final classification was evaluated against histologic diagnoses using leave-one-out cross-validation at a spectral level.

Results We compared Raman spectroscopy examination of colon specimens from four patients with UC and four patients without UC. A total of 801 spectra were recorded from colon specimens. We evaluated 100 spectra each from the mucosal and serosal surfaces of patients with UC and 260 spectra from the mucosal surface and 341 spectra from the serosal surface of the patients who did not have UC. For samples from the mucosal surface, the Raman analysis had a sensitivity of 82% and a specificity of 89%. For samples from the serosal surface, Raman spectroscopy had a sensitivity of 87% and a specificity of 93%. When considering each tissue sample and deciding the diagnosis based on the majority of spectra from that sample, there were no errors in the diagnosis.

Conclusions Raman spectroscopy can distinguish UC from normal colon tissue rapidly and accurately. This technology offers the possibility of real-time diagnosis as well as the ability to study changes in UC-afflicted colon tissue that do not appear histologically.

 
  • References

  • 1 Danese S, Fiocchi C. Ulcerative colitis. N Engl J Med 2011; 365 (18) 1713-1725
  • 2 Gisbert JP, González-Lama Y, Maté J. Role of biological markers in inflammatory bowel disease [in Spanish]. Gastroenterol Hepatol 2007; 30 (3) 117-129
  • 3 Rodriguez LG, Lockett SJ, Holtom GR. Coherent anti-stokes Raman scattering microscopy: a biological review. Cytometry A 2006; 69 (8) 779-791
  • 4 Movasaghi Z, Rehman S, Rehman IU. Raman spectroscopy of biological tissues. Appl Spectrosc Rev 2007; 42 (5) 493-541
  • 5 Lawson EE, Barry BW, Williams AC , et al. Biomedical applications of Raman spectroscopy. J Raman Spectrosc 1997; 28 (2–3) 111-117
  • 6 Reisner LA, Cao A, Pandya AK. An integrated software system for processing, analyzing, and classifying Raman spectra. Chemom Intell Lab Syst 2011; 105 (1) 83-90
  • 7 Socrates G. Infrared and Raman Characteristic Group Frequencies: Tables and Charts. West Sussex, England: John Wiley & Sons Ltd; 2004
  • 8 Zhang SZ, Zhao XH, Zhang DC. Cellular and molecular immunopathogenesis of ulcerative colitis. Cell Mol Immunol 2006; 3 (1) 35-40
  • 9 Hanauer SB. Inflammatory bowel disease. N Engl J Med 1996; 334 (13) 841-848
  • 10 Gibson PR, Muir JG. Reinforcing the mucus: a new therapeutic approach for ulcerative colitis?. Gut 2005; 54 (7) 900-903
  • 11 Mehta SJ, Silver AR, Lindsay JO. Review article: strategies for the management of chronic unremitting ulcerative colitis. Aliment Pharmacol Ther 2013; 38 (2) 77-97
  • 12 van Schaik FD, Oldenburg B, Offerhaus GJ , et al. Role of immunohistochemical markers in predicting progression of dysplasia to advanced neoplasia in patients with ulcerative colitis. Inflamm Bowel Dis 2012; 18 (3) 480-488
  • 13 Yun J, Xu CT, Pan BR. Epidemiology and gene markers of ulcerative colitis in the Chinese. World J Gastroenterol 2009; 15 (7) 788-803
  • 14 Roy HK, Backman V. Spectroscopic applications in gastrointestinal endoscopy. Clin Gastroenterol Hepatol 2012; 10 (12) 1335-1341
  • 15 Grivennikov SI, Greten FR, Karin M. Immunity, inflammation, and cancer. Cell 2010; 140 (6) 883-899
  • 16 Kiesslich R, Neurath MF. Endoscopic detection of early lower gastrointestinal cancer. Best Pract Res Clin Gastroenterol 2005; 19 (6) 941-961