PDF herunterladen
Drug Res (Stuttg) 2015; 65(10): 535-539
DOI: 10.1055/s-0034-1390459
Original Article
© Georg Thieme Verlag KG Stuttgart · New York

Increased Angiogenesis and Decreased Programmed Cell Death Increases the Risk of Uterine Cervical Cancer

Z.-l. Qin
1   Department of Gynaecology, Jinan Maternity and Child Care Hospital, Shandong, China
,
X.-x. Zheng
1   Department of Gynaecology, Jinan Maternity and Child Care Hospital, Shandong, China
,
Y.-h. Xu
1   Department of Gynaecology, Jinan Maternity and Child Care Hospital, Shandong, China
› Institutsangaben
Weitere Informationen

Publikationsverlauf

received 02. September 2014

accepted 12. September 2014

Publikationsdatum:
21. Oktober 2014 (online)

Auch verfügbar auf
    Lizenzen und Reprints

Abstract

Introduction: Cervical cancer is one of the most common female malignancies and leading cause for high mortality rate. In the present study we made an attempt to determine the extent of angiogenesis, apoptosis, accumulation of mutant p53 protein, cell proliferation rate in the uterine cervical cancer tissues.

Materials and Methods: Cervical cancer samples were obtained from patients and they were subjected to PCR analysis and immunocytochemistry.

Results: A total of 30 cervical cancer tissue samples were analyzed, by PCR, we found 25 collected cervical cancer samples showed HPV-16 and E6 positive. Further, we observed the increased CD34 expression was associated with HPV-16 and E6 positive cancer tissues when compared to the corresponding control tissues. This elevated level of CD34 confirms the increased extent of angiogenesis in cervical cancer tissues. Further by immunocytochemistry we have demonstrated that the rate of apoptosis is reduced, over expression of bcl-2, Ki 67 and thus increases rate of cell proliferation.

Discussion: Therefore, our data suggest that development of new anticancer or antiviral drugs could efficiently compromise the HPV-16 mediated angiogenesis and reduced apoptosis in cervical cancer and thus will improve the survival rate of patients.

Key words

cervical cancer - human papilloma virus - angiogenesis - apoptosis - cell proliferation
 

  • References

  • 1 Bosch FX, Manos MM, Muñoz N et al. Prevalence of human papillomavirus in cervical cancer: a worldwide perspective. International Biological Study on Cervical Cancer (IBSCC) Study Group. J Natl Cancer Inst 1995; 87: 796-802
    CrossrefPubMedSuche in Google Scholar
  • 2 Hanahan D, Folkman J. Patterns and emerging mechanisms of angiogenic switch during tumorigenesis. Cell 1996; 86: 353-364
    CrossrefPubMedSuche in Google Scholar
  • 3 Folkman J. The role of angiogenesis in tumor growth. Seminars in Cancer Biology 1992; 3: 65-67
    PubMedSuche in Google Scholar
  • 4 Guchelaar HJ, Vermes A, Vermes I et al. Apoptosis. Molecular mechanisms and implications for cancer chemotherapy. Pharmacy World and Science 1997; 19: 119-125
    CrossrefPubMedSuche in Google Scholar
  • 5 Lakshmi S, Nair BS, Jayaprakash PG et al. P53 protein and tumorigenesis in the uterine cervix. Gen Diag Pathol 1997; 142: 281-287
    PubMedSuche in Google Scholar
  • 6 Pillai R, Halabi S, McKalip A et al. The Presence of Human Papillomavirus 16/18 E6, p53 and Bcl-2 protein in cervicovaginal smears from patients with invasive cervical cancer. Cancer Epidemiol, Biomark Prevent 1996; 5: 329-335
    PubMedSuche in Google Scholar
  • 7 Pan H, Yin C, Van-Dyke T. Apoptosis and cancer mechanisms. Cancer Surveys 1997; 29: 305-327
    PubMedSuche in Google Scholar
  • 8 Ravi D, Ramdas K, Mathew BS et al. Angiogenesis during tumor progression in the oral cavity is related to reduced apoptosis and high tumor cell proliferation. Eur J Cancer Part II. Oral Oncol 1998; 34: 543-548
    CrossrefPubMedSuche in Google Scholar
  • 9 Pillai MR, Kesari AL, Chellam VG et al. Spontaneous programmed cell death in infiltrating duct carcinoma: association with p53, bcl-2, hormone receptors and tumor proliferation. Pathol Res Pract 1998; 194: 549-557
    CrossrefPubMedSuche in Google Scholar
  • 10 Nair P, Gangadevi T, Jayaprakash PG et al. Increased Angiogenesis in the Uterine Cervix Associated with Human Papillomavirus Infection. Pathol Res Pract 1991; 195: 163-169
    PubMedSuche in Google Scholar
  • 11 Bosserhoff AK, Glabl A, Stolz W et al. Detection of telomerase activity in skin, melanocytic nevi and melanoma by telomerase PCR ELISA. Biochemica 1997; 3: 16-18
    PubMedSuche in Google Scholar
  • 12 Kaufman RH, Adam E, Kenogle J et al. Relevance of human papillomavirus screening in the management of cervical intraepithelial neoplasia. Am J Obstet Gynecol 1997; 176: 87-92
    CrossrefPubMedSuche in Google Scholar
  • 13 Zehbe I, Wilander E. Human papillomavirus infection and invasive cervical neoplasia. A study of prevalence and morphology. J Pathol 1997; 181: 270-275
    CrossrefPubMedSuche in Google Scholar
  • 14 Das BC, Sharma JK, Gobalakrishna V et al. A high frequency of human papillomavirus DNA sequences in cervical carcinomas of Indian women as revealed by Southern blot hybridization and polymerase chain reaction. J Med Virol 1992; 36: 239-245
    CrossrefPubMedSuche in Google Scholar
  • 15 Kubbutat MHG, Vousden KH. Role of E6 and E7 oncoproteins in anogenital malignancies. Semin Virol 1996; 7: 295-304
    CrossrefPubMedSuche in Google Scholar
  • 16 Nair P, Jayaprakash PG, Nair MK et al. Telomerase, p53 and human papillomavirus infection in the uterine cervix. Acta Oncol 2000; 39: 65-70
    CrossrefPubMedSuche in Google Scholar
  • 17 Nair P, Nair KM, Jayaprakash PG et al. Decreased Programmed Cell Death in the Uterine Cervix Associated with High Risk Human Papillomavirus Infection. Pathol Oncol Res 1999; 5: 95-103
    CrossrefPubMedSuche in Google Scholar
  • 18 Dameron KM, Volpert OV, Tainsky MA et al. Control of angiogenesis in fibroblasts by p53 regulation of thrombospondin-l. Science 1994; 265: 1582-1584
    CrossrefPubMedSuche in Google Scholar