Abstract
Introduction: Cervical cancer is one of the most common female malignancies and leading cause
for high mortality rate. In the present study we made an attempt to determine the
extent of angiogenesis, apoptosis, accumulation of mutant p53 protein, cell proliferation
rate in the uterine cervical cancer tissues.
Materials and Methods: Cervical cancer samples were obtained from patients and they were subjected to PCR
analysis and immunocytochemistry.
Results: A total of 30 cervical cancer tissue samples were analyzed, by PCR, we found 25 collected
cervical cancer samples showed HPV-16 and E6 positive. Further, we observed the increased
CD34 expression was associated with HPV-16 and E6 positive cancer tissues when compared
to the corresponding control tissues. This elevated level of CD34 confirms the increased
extent of angiogenesis in cervical cancer tissues. Further by immunocytochemistry
we have demonstrated that the rate of apoptosis is reduced, over expression of bcl-2,
Ki 67 and thus increases rate of cell proliferation.
Discussion: Therefore, our data suggest that development of new anticancer or antiviral drugs
could efficiently compromise the HPV-16 mediated angiogenesis and reduced apoptosis
in cervical cancer and thus will improve the survival rate of patients.
Key words
cervical cancer - human papilloma virus - angiogenesis - apoptosis - cell proliferation
