Antioxidative Activity of Flavonoids from Abrus cantoniensis against Ethanol-Induced Gastric Ulcer in Mice

Hui Li; Zi-Jing Song; Yan-Ping Dai; Su-Li Zhang; Xin He; Chang-Run Guo; Wen-Jun Zhang; Jiao-Ying Wang; Chun-Feng Zhang; Chong-Zhi Wang; Chun-Su Yuan

doi:10.1055/s-0035-1546080

Planta Medica, Inhaltsverzeichnis

Planta Med 2015; 81(10): 784-790
DOI: 10.1055/s-0035-1546080

Biological and Pharmacological Activity

Original Papers

Georg Thieme Verlag KG Stuttgart · New York

Antioxidative Activity of Flavonoids from Abrus cantoniensis against Ethanol-Induced Gastric Ulcer in Mice

Hui Li^*

¹Laboratory of Traditional Chinese Medicine College, China Pharmaceutical University, Nanjing, JS, China

,

Zi-Jing Song^*

¹Laboratory of Traditional Chinese Medicine College, China Pharmaceutical University, Nanjing, JS, China

,

Yan-Ping Dai

¹Laboratory of Traditional Chinese Medicine College, China Pharmaceutical University, Nanjing, JS, China

,

Su-Li Zhang

¹Laboratory of Traditional Chinese Medicine College, China Pharmaceutical University, Nanjing, JS, China

,

Xin He

²School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, JS, China

,

Chang-Run Guo

¹Laboratory of Traditional Chinese Medicine College, China Pharmaceutical University, Nanjing, JS, China

,

Wen-Jun Zhang

¹Laboratory of Traditional Chinese Medicine College, China Pharmaceutical University, Nanjing, JS, China

,

Jiao-Ying Wang

¹Laboratory of Traditional Chinese Medicine College, China Pharmaceutical University, Nanjing, JS, China

,

Chun-Feng Zhang

¹Laboratory of Traditional Chinese Medicine College, China Pharmaceutical University, Nanjing, JS, China

³Tang Center of Herbal Medicine Research and Department of Anesthesia & Critical Care, University of Chicago, Chicago, IL, USA

,

Chong-Zhi Wang

³Tang Center of Herbal Medicine Research and Department of Anesthesia & Critical Care, University of Chicago, Chicago, IL, USA

,

Chun-Su Yuan

³Tang Center of Herbal Medicine Research and Department of Anesthesia & Critical Care, University of Chicago, Chicago, IL, USA

› Institutsangaben

Abstract

The present study investigated the flavonoids from Abrus cantoniensis against ethanol-induced gastric ulcers in mice. The flavonoids from A. cantoniensis were extracted with ethanol and purified by macroporous resin and polyamide. The 2,2-diphenyl-1-picrylhydrazyl assay was used to measure the antioxidative activities in vitro. The ethanol-induced ulcer mouse model was used to evaluate the gastroprotective activities of the flavonoids from A. cantoniensis. In addition, a method was established to ensure accuracy for animal ulcer evaluation. The flavonoids from A. cantoniensis showed a strong free radical scavenging capacity with an IC₅₀ of 43.83 µg/mL in the 2,2-diphenyl-1-picrylhydrazyl assay. At doses between 28.16–112.67 mg/kg, the flavonoids conspicuously reduced the ulcer index in ethanol-induced mice (p < 0.001). Significant differences were found in the levels of superoxide dismutase, catalase, glutathione, and myeloperoxidase in the stomach tissues between the flavonoids from the A. cantoniensis groups and the ethanol control group. The gastroprotective effect of the flavonoids from A. cantoniensis could be due to its antioxidative activity of the defensive mechanism. The data revealed that the flavonoids from A. cantoniensis could be a potential therapeutic agent for gastric ulcer prevention and treatment.

Key words

Abrus cantoniensis - Leguminosae - flavonoid - antioxidant - gastroprotection - Image Pro-Plus

Volltext

Referenzen

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