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DOI: 10.1055/s-0035-1546256
Seco-ursane-type Triterpenoids from Salvia urmiensis with Apoptosis-inducing Activity
Publikationsverlauf
received 31. Januar 2015
revised 15. April 2015
accepted 02. Juni 2015
Publikationsdatum:
07. August 2015 (online)
![](https://www.thieme-connect.de/media/plantamedica/201514/lookinside/thumbnails/10-1055-s-0035-1546256_pmz0120-1.jpg)
Abstract
Fractionation of an acetone extract of the aerial parts of Salvia urmiensis led to the isolation of a new (1) and a known (2) E-seco-ursane-type triterpenoid, together with four other known compounds. Their structures were established by 1D and 2D nuclear magnetic resonance as well as high-resolution electrospray ionization mass spectrometry. The effect of compounds 1 and 2 on cell viability of HeLa and HepG2 cells was investigated with the MTT assay. We also report the mechanism of action of compound 2 as a potential anticancer agent in HeLa cells. Bcl-2, Bax, and caspases signaling pathway expression in HeLa cells was analyzed. HeLa cells treated with compound 2 were assayed for the cleavage of poly-(ADP-ribose)-polymerase and DNA fragmentation resulting in nuclear shrinkage. Taken together, these results suggest that treatment of HeLa cells with compound 2 can induce apoptosis by regulating Bcl-2 family members and by suppressing caspase cascade activation.
Key words
Salvia urmiensis - Lamiaceae - isolation - structure elucidation - cytotoxic activity - apoptosis-
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