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J Knee Surg 2016; 29(03): 242-247
DOI: 10.1055/s-0035-1549022
Original Article
Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

Identification of Synovial Fluid Biomarkers for Knee Osteoarthritis and Correlation with Radiographic Assessment

Farrah Monibi
1   Comparative Orthopaedic Laboratory, University of Missouri, Columbia, Missouri
,
Brandon L. Roller
2   Arthrex, Inc., Naples, Florida
,
Aaron Stoker
1   Comparative Orthopaedic Laboratory, University of Missouri, Columbia, Missouri
,
Bridget Garner
4   Missouri Orthopaedic Institute, Department of Orthopaedic Surgery, University of Missouri, Columbia, Missouri
,
Sonny Bal
5   Department of Orthopaedic Surgery, University of Missouri, Orthopaedic Institute Columbia, Missouri
,
James L. Cook
1   Comparative Orthopaedic Laboratory, University of Missouri, Columbia, Missouri
4   Missouri Orthopaedic Institute, Department of Orthopaedic Surgery, University of Missouri, Columbia, Missouri
› Author Affiliations
Further Information

Publication History

09 September 2014

10 February 2015

Publication Date:
30 April 2015 (online)

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Abstract

Osteoarthritis (OA) is a costly and debilitating condition that is typically not diagnosed early enough to prevent progression of disease. The purpose of this study was to evaluate synovial fluid from knees with and without OA for potential markers of joint inflammation and degradation and to correlate these findings with radiographic severity of disease. With Institutional Review Board approval, synovial fluid samples were collected before the patient undergoing total knee arthroplasty. Control knees (n = 3) were patients younger than 30 years of age with no history of anterior cruciate ligament, posterior cruciate ligament, or meniscal injury, and no surgical history for either knee. Weight-bearing, anterior–posterior radiographic views were used to determine radiographic OA severity using the modified Kellgren and Lawrence scale. Synovial fluid samples from 18 patients (21 knees) were analyzed using a multiplex assay. Matrix metalloproteinase (MMP)-1 (p < 0.001), interleukin (IL)-6 (p < 0.013), IL-8 (p < 0.024), and Chemokine (C-C motif) ligand 5 (CCL5) (p < 0.006) were significantly higher in the synovial fluid of OA patients compared with normal patients. The radiographic score was significantly higher in patients with OA compared with normal knees (p < 0.002). MMP-1 had a moderate positive correlation with MMP-2, IL-6, IL-8, and CCL5. IL-6 had a strong positive correlation with IL-8 and a moderate positive correlation with MMP-2. Monocyte chemotactic protein 1 had a moderate positive correlation with IL-6 and a strong positive correlation with IL-8. Radiographic scores had a strong positive correlation with IL-6 and IL-8 and a moderate positive correlation with MCP-1. These data provide novel and clinically relevant information for the investigation of synovial fluid biomarkers for knee OA.

Keywords

knee - synovial fluid - biomarkers - osteoarthritis - radiography

Note

This is the final in a series of four articles.


 

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