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DOI: 10.1055/s-0035-1554782
Cerebrospinal Fluid Selenium Concentrations in Pediatric Patients with Neurologic Disorders
Publication History
Publication Date:
03 July 2015 (online)
![](https://www.thieme-connect.de/media/10.1055-s-00029022/201501/lookinside/thumbnails/10-1055-s-0035-1554782_15004-1.jpg)
Abstract
We aimed to study the cerebrospinal fluid (CSF) selenium (Se) status in pediatric patients with neurologic disorders and to assess the relationship between CSF Se concentrations, proteins, glutathione peroxidase (GPx) activity, and patient clinical data. We analyzed CSF Se by inductively coupled plasma-mass spectrometry (ICP-MS) in 212 patients with several neurologic conditions. Student's t-test was applied to compare CSF Se values from patients with those of controls. Single and multiple correlation studies were applied to assess the association between the different variables. Nineteen patients presented with abnormal CSF Se concentrations when compared with our reference values established for the different groups of age (group 1: 1–30 days; 1.8–4.7 µg/L; group 2: 1–36 months; 0.68–3.0 µg/L; group 3: 4–18 years; 0.73–2.13 µg/L) associated with different clinical conditions. Ten had decreased CSF Se levels and nine had increased levels. A positive correlation between CSF Se and GPx activity (r = 0.586; p <0.001), as well as with total proteins (r = 0.387; p <0.001) and with albumin (r = 0.898; p <0.001), was observed. Impaired Se concentrations affected 9% of patients with neurologic dysfunction. The positive correlation observed between CSF Se and CSF albumin values suggests that this protein markedly contributes to total Se concentrations. Also, the positive correlation observed between CSF Se values and GPx activity suggests that it may be a biomarker for antioxidant status in the CSF.
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References
- 1 Burk RF, Hill KE, Motley AK. Selenoprotein metabolism and function: evidence for more than one function for selenoprotein P. J Nutr 2003; 133 (5) (Suppl. 01) 1517S-1520S
- 2 Richardson DR. More roles for selenoprotein P: local selenium storage and recycling protein in the brain. Biochem J 2005; 386 (Pt 2) e5-e7
- 3 Tondo M, Moreno J, Casado M , et al. Selenium concentration in cerebrospinal fluid samples from a paediatric population. Neurochem Res 2010; 35 (8) 1290-1293
- 4 Chen J, Berry MJ. Selenium and selenoproteins in the brain and brain diseases. J Neurochem 2003; 86 (1) 1-12
- 5 Moghadaszadeh B, Beggs AH. Selenoproteins and their impact on human health through diverse physiological pathways. Physiology (Bethesda) 2006; 21: 307-315
- 6 Bräuer AU, Savaskan NE. Molecular actions of selenium in the brain: neuroprotective mechanisms of an essential trace element. Rev Neurosci 2004; 15 (1) 19-32
- 7 Alimonti A, Bocca B, Pino A, Ruggieri F, Forte G, Sancesario G. Elemental profile of cerebrospinal fluid in patients with Parkinson's disease. J Trace Elem Med Biol 2007; 21 (4) 234-241
- 8 Forte G, Bocca B, Senofonte O , et al. Trace and major elements in whole blood, serum, cerebrospinal fluid and urine of patients with Parkinson's disease. J Neural Transm 2004; 111 (8) 1031-1040
- 9 Meseguer I, Molina JA, Jiménez-Jiménez FJ , et al. Cerebrospinal fluid levels of selenium in patients with Alzheimer's disease. J Neural Transm 1999; 106 (3-4) 309-315
- 10 Berr C. Oxidative stress and cognitive impairment in the elderly. J Nutr Health Aging 2002; 6 (4) 261-266
- 11 Gerhardsson L, Lundh T, Minthon L, Londos E. Metal concentrations in plasma and cerebrospinal fluid in patients with Alzheimer's disease. Dement Geriatr Cogn Disord 2008; 25 (6) 508-515
- 12 Dumitrescu AM, Liao XH, Abdullah MS , et al. Mutations in SECISBP2 result in abnormal thyroid hormone metabolism. Nat Genet 2005; 37 (11) 1247-1252
- 13 Ashrafi MR, Shams S, Nouri M , et al. A probable causative factor for an old problem: selenium and glutathione peroxidase appear to play important roles in epilepsy pathogenesis. Epilepsia 2007; 48 (9) 1750-1755
- 14 Schoenmakers E, Agostini M, Mitchell C , et al. Mutations in the selenocysteine insertion sequence-binding protein 2 gene lead to a multisystem selenoprotein deficiency disorder in humans. J Clin Invest 2010; 120 (12) 4220-4235
- 15 Tondo M, Málaga I, O'Callaghan M , et al. Biochemical parameters to assess choroid plexus dysfunction in Kearns-Sayre syndrome patients. Mitochondrion 2011; 11 (6) 867-870
- 16 Ormazabal A, García-Cazorla A, Fernández Y, Fernández-Alvarez E, Campistol J, Artuch R. HPLC with electrochemical and fluorescence detection procedures for the diagnosis of inborn errors of biogenic amines and pterins. J Neurosci Methods 2005; 142 (1) 153-158
- 17 Heitland P, Köster HD. Biomonitoring of 37 trace elements in blood samples from inhabitants of northern Germany by ICP-MS. J Trace Elem Med Biol 2006; 20 (4) 253-262
- 18 Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 1967; 70 (1) 158-169
- 19 Justel A, Peña D, Zamar R. A multivariate Kolomogorov-Smirnov test of goodness of fit. Stat Probab Lett 1997; 35 (3) 251-259
- 20 Choi SC. Tests of equality of dependent correlation coefficients. Biometrika 1977; 64 (3) 645-647
- 21 Fisher Box J. Guinness, Gosset, Fisher and small samples. Statist Sci 1987; 2 (1) 45-52
- 22 Plackett RL. Karl Pearson and the Chi-squared test. Int Stat Rev 1983; 51 (1) 59-72
- 23 Solovyev N, Berthele A, Michalke B. Selenium speciation in paired serum and cerebrospinal fluid samples. Anal Bioanal Chem 2013; 405 (6) 1875-1884
- 24 Steinbrenner H, Sies H. Selenium homeostasis and antioxidant selenoproteins in brain: implications for disorders in the central nervous system. Arch Biochem Biophys 2013; 536 (2) 152-157
- 25 van Bakel MM, Printzen G, Wermuth B, Wiesmann UN. Antioxidant and thyroid hormone status in selenium-deficient phenylketonuric and hyperphenylalaninemic patients. Am J Clin Nutr 2000; 72 (4) 976-981