Antinociceptive and Anti-inflammatory Activities of the Methanolic Extract from the Stem Bark of Lophanthera lactescens

Gabriela Carmelinda Martins dos Santos; Renata Duarte Fernandes; Tarcila Rocha Barros; Heber dos Santos Abreu; Luciano Ramos Suzart; Mário Geraldo de Carvalho; Raimundo Braz Filho; Bruno Guimarães Marinho

doi:10.1055/s-0035-1557837

Planta Medica, Inhaltsverzeichnis

Planta Med 2015; 81(18): 1688-1696
DOI: 10.1055/s-0035-1557837

Biological and Pharmacological Activitiy

Original Papers

Georg Thieme Verlag KG Stuttgart · New York

Antinociceptive and Anti-inflammatory Activities of the Methanolic Extract from the Stem Bark of Lophanthera lactescens

Autor*innen

Gabriela Carmelinda Martins dos Santos

¹Laboratório de Farmacologia, Departamento de Ciências Fisiológicas, Instituto de Biologia, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brazil
Renata Duarte Fernandes

²Laboratório de Produtos Naturais, Departamento de Química, Instituto de Ciências Exatas, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brazil
Tarcila Rocha Barros

²Laboratório de Produtos Naturais, Departamento de Química, Instituto de Ciências Exatas, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brazil
Heber dos Santos Abreu

³Instituto de Floresta, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brazil
Luciano Ramos Suzart

²Laboratório de Produtos Naturais, Departamento de Química, Instituto de Ciências Exatas, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brazil
Mário Geraldo de Carvalho

²Laboratório de Produtos Naturais, Departamento de Química, Instituto de Ciências Exatas, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brazil
Raimundo Braz Filho

²Laboratório de Produtos Naturais, Departamento de Química, Instituto de Ciências Exatas, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brazil
Bruno Guimarães Marinho

¹Laboratório de Farmacologia, Departamento de Ciências Fisiológicas, Instituto de Biologia, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brazil

Abstract

Lophanthera lactescens is a medicinal plant commonly used in traditional medicine to relieve fever and pain in inflammatory processes. In the present study, the in vivo antinociceptive and anti-inflammatory effects of the methanolic extract from L. lactescens have been investigated. Antinociceptive activity was evaluated through writhing, formalin, and tail flick tests, while the anti-inflammatory activity was evaluated through paw oedema and air pouch tests in mice. A phytochemical analysis was performed. The extract produced significant inhibition on nociception induced by acetic acid-induced abdominal writhing, formalin, and tail flick tests, and on inflammation induced by oedema and air pouch tests. The previous administration of atropine and glibenclamide reduced the antinociceptive effect produced by the methanolic extract from L. lactescens on the tail flick test in 89 % and 66 %, respectively. The methanolic extract had no significant effect in the open field test. No intoxication symptoms were observed in the animals administered orally at increasing doses up to 2000 mg/kg. The methanolic extract from the stem bark of L. lactescens possesses antinociceptive properties on models of acute pain induced by chemical and thermal stimuli as well as in models of inflammation and further suggests that this anti-inflammatory activity might involve inhibition of the proinflammatory cytokines, and the antinociceptive activity might involve participation of the cholinergic system and adenosine triphosphate-dependent K+ channel.

Key words

Lophanthera lactescens - Malpighiaceae - cytokines - methanolic extract - mice - nociception

Volltext

Referenzen

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