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Planta Med 2015; 81(18): 1719-1726
DOI: 10.1055/s-0035-1557867
Natural Product Chemistry
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Poly(N-Isopropylacrylamide)-co-Acrylamide Hydrogels for the Controlled Release of Bromelain from Agroindustrial Residues of Ananas comosus

Fernanda Croisfelt
1   Genetics and Biochemistry Institute, UFU, Uberlândia, MG, Brazil
,
Bianca C. Martins
1   Genetics and Biochemistry Institute, UFU, Uberlândia, MG, Brazil
,
Robson Rescolino
1   Genetics and Biochemistry Institute, UFU, Uberlândia, MG, Brazil
,
Diego F. Coelho
,
Beatriz Zanchetta
2   School of Chemical Engineering, Unicamp, Campinas, SP, Brazil
,
Priscila G. Mazzola
3   Faculty of Pharmaceutical Sciences, Unicamp, Campinas, SP, Brazil
,
Luis Ricardo Goulart
1   Genetics and Biochemistry Institute, UFU, Uberlândia, MG, Brazil
,
Adalberto Jr. Pessoa
4   School of Pharmaceutical Sciences, USP, São Paulo, SP, Brazil
,
Elias B. Tambourgi
2   School of Chemical Engineering, Unicamp, Campinas, SP, Brazil
,
Edgar Silveira
1   Genetics and Biochemistry Institute, UFU, Uberlândia, MG, Brazil
› Author Affiliations
Further Information

Publication History

received 11 March 2015
revised 07 July 2015

accepted 14 July 2015

Publication Date:
19 August 2015 (online)

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Abstract

This works reports the purification of bromelain extracted from Ananas comosus industrial residues by ethanol purification, its partial characterization from the crude extract as well as the ethanol purified enzyme, and its application onto poly(N-isopropylacrylamide)-co-acrylamide hydrogels. Bromelain was recovered within the 30–70 % ethanol fraction, which achieved a purification factor of 3.12-fold, and yielded more than 90 % of its initial activity. The resulting purified bromelain contained more than 360 U · mg−1, with a maximum working temperature of 60 °C and pH of 8.0. Poly(N-isopropylacrylamide)-co-acrylamide hydrogels presented a swelling rate of 125 %, which was capable of loading 56 % of bromelain from the solution, and was able to release up to 91 % of the retained bromelain. Ethanol precipitation is suitable for bromelain recovery and application onto poly(N-isopropylacrylamide)-co-acrylamide hydrogels based on its processing time and the applied ethanol prices.

Key words

Ananas comosus - Bromeliaceae - N-isopropylpolyacrylamide - hydrogels - bromelain - ethanol purification - controlled release
 

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