Poly(N-Isopropylacrylamide)-co-Acrylamide Hydrogels for the Controlled Release of Bromelain from Agroindustrial Residues of Ananas comosus

Fernanda Croisfelt; Bianca C. Martins; Robson Rescolino; Diego F. Coelho; Beatriz Zanchetta; Priscila G. Mazzola; Luis Ricardo Goulart; Adalberto Jr. Pessoa; Elias B. Tambourgi; Edgar Silveira

doi:10.1055/s-0035-1557867

Planta Medica, Table of Contents

Planta Med 2015; 81(18): 1719-1726
DOI: 10.1055/s-0035-1557867

Natural Product Chemistry

Original Papers

Georg Thieme Verlag KG Stuttgart · New York

Poly(N-Isopropylacrylamide)-co-Acrylamide Hydrogels for the Controlled Release of Bromelain from Agroindustrial Residues of Ananas comosus

Authors

Fernanda Croisfelt

¹Genetics and Biochemistry Institute, UFU, Uberlândia, MG, Brazil
Bianca C. Martins

¹Genetics and Biochemistry Institute, UFU, Uberlândia, MG, Brazil
Robson Rescolino

¹Genetics and Biochemistry Institute, UFU, Uberlândia, MG, Brazil
Diego F. Coelho
Beatriz Zanchetta

²School of Chemical Engineering, Unicamp, Campinas, SP, Brazil
Priscila G. Mazzola

³Faculty of Pharmaceutical Sciences, Unicamp, Campinas, SP, Brazil
Luis Ricardo Goulart

¹Genetics and Biochemistry Institute, UFU, Uberlândia, MG, Brazil
Adalberto Jr. Pessoa

⁴School of Pharmaceutical Sciences, USP, São Paulo, SP, Brazil
Elias B. Tambourgi

²School of Chemical Engineering, Unicamp, Campinas, SP, Brazil
Edgar Silveira

¹Genetics and Biochemistry Institute, UFU, Uberlândia, MG, Brazil

Abstract

This works reports the purification of bromelain extracted from Ananas comosus industrial residues by ethanol purification, its partial characterization from the crude extract as well as the ethanol purified enzyme, and its application onto poly(N-isopropylacrylamide)-co-acrylamide hydrogels. Bromelain was recovered within the 30–70 % ethanol fraction, which achieved a purification factor of 3.12-fold, and yielded more than 90 % of its initial activity. The resulting purified bromelain contained more than 360 U · mg⁻¹, with a maximum working temperature of 60 °C and pH of 8.0. Poly(N-isopropylacrylamide)-co-acrylamide hydrogels presented a swelling rate of 125 %, which was capable of loading 56 % of bromelain from the solution, and was able to release up to 91 % of the retained bromelain. Ethanol precipitation is suitable for bromelain recovery and application onto poly(N-isopropylacrylamide)-co-acrylamide hydrogels based on its processing time and the applied ethanol prices.

Key words

Ananas comosus - Bromeliaceae - N-isopropylpolyacrylamide - hydrogels - bromelain - ethanol purification - controlled release

Full Text

References

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