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J Neurol Surg A Cent Eur Neurosurg 2015; 76(06): 466-472
DOI: 10.1055/s-0035-1558416
Original Article
Georg Thieme Verlag KG Stuttgart · New York

An Interlaminotomy New Zealand White Rabbit Model to Evaluate Novel Epidural Strategies

Edin Nevzati
1   Department of Neurosurgery, Kantonsspital Aarau, Aarau, Switzerland
2   NeuroLab, Department of Clinical Research, University of Bern, Bern, Switzerland
,
Jehuda Soleman
1   Department of Neurosurgery, Kantonsspital Aarau, Aarau, Switzerland
2   NeuroLab, Department of Clinical Research, University of Bern, Bern, Switzerland
,
Salome Aglaia Schöpf
1   Department of Neurosurgery, Kantonsspital Aarau, Aarau, Switzerland
2   NeuroLab, Department of Clinical Research, University of Bern, Bern, Switzerland
,
Daniel Coluccia
1   Department of Neurosurgery, Kantonsspital Aarau, Aarau, Switzerland
2   NeuroLab, Department of Clinical Research, University of Bern, Bern, Switzerland
,
Javier Fandino
1   Department of Neurosurgery, Kantonsspital Aarau, Aarau, Switzerland
2   NeuroLab, Department of Clinical Research, University of Bern, Bern, Switzerland
,
Serge Marbacher
1   Department of Neurosurgery, Kantonsspital Aarau, Aarau, Switzerland
2   NeuroLab, Department of Clinical Research, University of Bern, Bern, Switzerland
› Author Affiliations
Further Information

Publication History

06 May 2014

31 March 2015

Publication Date:
09 September 2015 (online)

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Abstract

Objective The New Zealand White (NZW) rabbit model is an established animal model for examining surgical methods to prevent epidural scar formation after spine surgery. As most approaches include complete laminectomy of the rabbit vertebra, this procedure is associated with high morbidity and mortality rates. We examined a less invasive technique, the microsurgical interlaminotomy, for testing epidural substance application in the rabbit spine.

Methods Surgery was performed in the cadaver rabbit spine to evaluate the approach before performing it in NZW rabbits. All surgical procedures were performed under an operation microscope. Female rabbits with a mean weight of 4770 g ± 240 g were used. Neurologic symptoms were analyzed based on predefined scores. After resection of the spinal process, the caudal part of the upper lamina was resected using a drill and a 1-mm Kerrison punch. The yellow ligament was resected resulting in a dural exposure of ∼ 5 × 10 mm.

Results Eight pilot interlaminotomies were performed on three cadaveric spines to establish the surgical approach. Twenty-one NZW rabbits were then operated on using the interlaminotomy model. Three rabbits (14.3%) died during surgery due to anesthesia-related complications. Two rabbits (9.5%) showed partial paresis of the lower extremities and one (4.8%) a complete paraplegia. The remaining 15 rabbits (71.4%) had an uneventful recovery without neurologic symptoms. The mean surgical duration was 88 +/− 28 minutes.

Conclusion The rabbit interlaminotomy model is associated with few neurologic deficits and a relatively short operating time.

Keywords

scar - laminectomy - New Zealand white rabbits - animal - model
 

  • References

  • 1 Robertson JT. Role of peridural fibrosis in the failed back: a review. Eur Spine J 1996; 5 (Suppl. 01) S2-S6
    CrossrefPubMedSearch in Google Scholar
  • 2 Benoist M, Ficat C, Baraf P, Cauchoix J. Postoperative lumbar epiduro-arachnoiditis. Diagnostic and therapeutic aspects. Spine 1980; 5 (5) 432-436
    CrossrefPubMedSearch in Google Scholar
  • 3 Cauchoix J, Ficat C, Girard B. Repeat surgery after disc excision. Spine 1978; 3 (3) 256-259
    CrossrefPubMedSearch in Google Scholar
  • 4 Sandoval MA, Hernandez-Vaquero D. Preventing peridural fibrosis with nonsteroidal anti-inflammatory drugs. Eur Spine J 2008; 17 (3) 451-455
    CrossrefPubMedSearch in Google Scholar
  • 5 Kaya M, Yildirim CH, Kosemehmetoglu K , et al. Alpha-lipoic acid reduces peridural fibrosis after laminectomy of lumbar vertebrae in rabbits. Acta Neurochir (Wien) 2012; 154 (7) 1241-1245
    CrossrefPubMedSearch in Google Scholar
  • 6 Rodgers KE, Robertson JT, Espinoza T , et al. Reduction of epidural fibrosis in lumbar surgery with Oxiplex adhesion barriers of carboxymethylcellulose and polyethylene oxide. Spine J 2003; 3 (4) 277-283 ; discussion 284
    CrossrefPubMedSearch in Google Scholar
  • 7 Langenskiöld A, Kiviluoto O. Prevention of epidural scar formation after operations on the lumbar spine by means of free fat transplants. A preliminary report. Clin Orthop Relat Res 1976; (115) 92-95
    PubMedSearch in Google Scholar
  • 8 Bora H, Aykol SV, Akyürek N, Akmansu M, Ataoglu O. Inhibition of epidural scar tissue formation after spinal surgery: external irradiation vs. spinal membrane application. Int J Radiat Oncol Biol Phys 2001; 51 (2) 507-513
    CrossrefPubMedSearch in Google Scholar
  • 9 Alkalay RN, Kim DH, Urry DW, Xu J, Parker TM, Glazer PA. Prevention of postlaminectomy epidural fibrosis using bioelastic materials. Spine 2003; 28 (15) 1659-1665
    PubMedSearch in Google Scholar
  • 10 Robertson JT, Meric AL, Dohan Jr FC, Schweitzer JB, Wujek JR, Ahmad S. The reduction of postlaminectomy peridural fibrosis in rabbits by a carbohydrate polymer. J Neurosurg 1993; 79 (1) 89-95
    CrossrefPubMedSearch in Google Scholar
  • 11 Cokluk C, Aydi K. Experimental rabbit hemilaminotomy model in the evaluation of peridural fibrosis: a minimally invasive peridural fibrosis model. Minim Invasive Neurosurg 2005; 48 (4) 235-239
    Thieme ConnectPubMedSearch in Google Scholar
  • 12 Palumbo M, Valdes M, Robertson A, Sheikh S, Lucas P. Posterolateral intertransverse lumbar arthrodesis in the New Zealand White rabbit model: I. Surgical anatomy. Spine J 2004; 4 (3) 287-292
    CrossrefPubMedSearch in Google Scholar
  • 13 Valdes M, Palumbo M, Appel AJ, McAllister S, Ehrlich M. Posterolateral intertransverse lumbar arthrodesis in the New Zealand White rabbit model: II. Operative technique. Spine J 2004; 4 (3) 293-299
    CrossrefPubMedSearch in Google Scholar
  • 14 Hiraizumi Y, Fujimaki E, Tachikawa T. Long-term morphology of spastic or flaccid muscles in spinal cord-transected rabbits. Clin Orthop Relat Res 1990; (260) 287-296
    PubMedSearch in Google Scholar
  • 15 Nihei A, Hagiwara K, Kikuchi M, Yashiro T, Hoshino Y. Histological investigation of rabbit ligamentum flavum with special reference to differences in spinal levels. Anat Sci Int 2003; 78 (3) 162-167
    CrossrefPubMedSearch in Google Scholar
  • 16 Basso DM, Beattie MS, Bresnahan JC. A sensitive and reliable locomotor rating scale for open field testing in rats. J Neurotrauma 1995; 12 (1) 1-21
    CrossrefPubMedSearch in Google Scholar
  • 17 Barberá J, Gonzalez J, Esquerdo J, Broseta J, Barcia-Salorio JL. Prophylaxis of the laminectomy membrane. An experimental study in dogs. J Neurosurg 1978; 49 (3) 419-424
    CrossrefPubMedSearch in Google Scholar
  • 18 LaRocca H, Macnab I. The laminectomy membrane. Studies in its evolution, characteristics, effects and prophylaxis in dogs. J Bone Joint Surg Br 1974; 56B (3) 545-550
    PubMedSearch in Google Scholar
  • 19 Preul MC, Campbell PK, Garlick DS, Spetzler RF. Application of a new hydrogel dural sealant that reduces epidural adhesion formation: evaluation in a large animal laminectomy model. J Neurosurg Spine 2010; 12 (4) 381-390
    CrossrefPubMedSearch in Google Scholar
  • 20 Colak A, Bavbek M, Aydin NE, Renda N, Açikgöz B. Effect of CO2 laser on spinal epidural fibrosis. Acta Neurochir (Wien) 1996; 138 (2) 162-166
    CrossrefPubMedSearch in Google Scholar
  • 21 Robertson JT, Soble-Smith J, Powers N, Nelson PA. Prevention of cerebrospinal fistulae and reduction of epidural scar with new surgical hemostat device in a porcine laminectomy model. Spine 2003; 28 (19) 2298-2303
    CrossrefPubMedSearch in Google Scholar
  • 22 Gerszten PC, Moossy JJ, Bahri S, Kalend A, Martínez AJ. Inhibition of peridural fibrosis after laminectomy using low-dose external beam radiation in a rat model. Neurosurgery 1999; 44 (3) 597-602 ; discussion 602–603
    CrossrefPubMedSearch in Google Scholar
  • 23 Hinton Jr JL, Warejcka DJ, Mei Y , et al. Inhibition of epidural scar formation after lumbar laminectomy in the rat. Spine 1995; 20 (5) 564-570 ; discussion 579–580
    CrossrefPubMedSearch in Google Scholar
  • 24 Greenaway JB, Partlow GD, Gonsholt NL, Fisher KR. Anatomy of the lumbosacral spinal cord in rabbits. J Am Anim Hosp Assoc 2001; 37 (1) 27-34
    CrossrefPubMedSearch in Google Scholar
  • 25 Krüger J, Zeller W, Schottmann E. A simplified procedure for endotracheal intubation in rabbits. Lab Anim 1994; 28 (2) 176-177
    CrossrefPubMedSearch in Google Scholar
  • 26 Smith JC, Robertson LD, Auhll A, March TJ, Derring C, Bolon B. Endotracheal tubes versus laryngeal mask airways in rabbit inhalation anesthesia: ease of use and waste gas emissions. Contemp Top Lab Anim Sci 2004; 43 (4) 22-25
    PubMedSearch in Google Scholar
  • 27 Phaneuf LR, Barker S, Groleau MA, Turner PV. Tracheal injury after endotracheal intubation and anesthesia in rabbits. J Am Assoc Lab Anim Sci 2006; 45 (6) 67-72
    PubMedSearch in Google Scholar