Planta Med 2015; 81 - PM_184
DOI: 10.1055/s-0035-1565561

In vitro biological and toxicological evaluation of Lathyrus sp. extracts

K Polatoğlu 1, 2, G Göger 3, 4, HE Temel 5, F Demirci 4
  • 1Istanbul Kemerburgaz University, Faculty of Pharmacy, Department of Analytical Chemistry, Istanbul, Turkey
  • 2Near East University, Faculty of Pharmacy, Department of Analytical Chemistry, Mersin/Lefkose, Turkey
  • 3Anadolu University, Graduate School of Health Sciences, Pharmacognosy Department, Eskisehir, Turkey
  • 4Anadolu University, Faculty of Pharmacy, Pharmacognosy Department, Eskisehir, Turkey
  • 5Anadolu University, Faculty of Pharmacy, Department of Biochemisty, Eskisehir, Turkey

Lathyrus L. (Fabaceae) species are well known with their edible uses [1] as well as the notorious “lathyrism” diseases associated with the oxalyldiaminopropionic acid [2]. There is very limited information on the biological activities of this genus. The n-hexane, CHCl3, ethyl acetate and CH3OH extracts of Lathyrus species including L. sativus, L. aphaca, L. cicera, L. gorgonei, L. saxatalis, L. blepharicarpos var. cyprius and L. ochrus were tested for their DPPH scavenging, PRAP, phytotoxic, Vibrio fischerii toxicity, cytotoxicity against NIH/3T3 cell line, LOX enzyme inhibition and as well as antimicrobial activity against a selection of pathogens. The highest DPPH scavenging activity and PRAP activity was observed for the CH3OH extract of L. cicera 79.48 ± 0.79% (n = 5) and CHCl3 extract of L. blepharicarpos var. cyprius 678.48 ± 15.48 AU (n = 5; AU TLC-densitometry) respectively. Highest phytotoxic activity was observed for CHCl3 extract of L. sativus at 10 mg/mL application concentration 72.86 ± 21.14% (n = 3). The highest V. fischerii toxicity was observed in L. cicera CHCl3 extract after 60 min of application which produced 5 LU at the same conditions positive control CuSO4 produced 22 LU. The highest cytotoxicity against NIH/3T3 cell line was observed in L. ochrus ethyl acetate extract LC50 137.5 ± 3.5 µg/mL (n = 3). The highest LOX inhibition was observed in ethyl acetate extract of L. cicera which afforded 23.96% at 23 µg/mL concentration. All of the extracts produced growth inhibition against the tested microorganisms except for the CH3OH extracts that had no effect on C. albicans. The active principles in the extracts are currently under investigation.

References:

[1] Viney DE. An illustrated Flora of North Cyprus, 2nd edition. Koenigstein: Koeltz Publishing; 1994: 222 – 227

[2] Harrison FL., Nunn PB., Hill RR. Synthesis of α- and β-N-Oxalyl-L-α,β-diaminopropionic acids and their isolation from seeds of Lathyrus sativus. Phytochemistry 1977; 16: 1211 – 1215