Mangifera indica actives key the metabolic master switch enzymes SIRT-1 and AMPK

Emerging research suggests that physical activity and calorie restriction prevent age-associated metabolic disturbances by maintaining the function of mitochondria, the power plant of cells, and by activating the evolutionary conserved metabolic sensors sirtuin-1 (Sirt-1) and AMP-activated protein kinase (AMPK) [1 – 3].

Mangifera indica L. fruit preparation, used in this study, is a 100% pure mango fruit powder obtained from fruits harvested in India at a special degree of ripeness. The fruits are characterized by a higher level of secondary plant ingredients Mangiferin and a lower level of sugar compared to fully ripe fruits.

Activation of human recombinant SIRT1 isoenzyme was investigated using both, a fluorescence and a luminescence assay. Activation of AMPK was investigated in HepaRG™ cells. AMPK activation was analyzed by detection and quantification of AMPK protein phosphorylated at threonine residue 172.

At a concentration of 500 µg/mL an SIRT1 activation of 30% was detected. The positive control resveratrol showed activation of 50%. Further, at a concentration of 300 µg/mL the Mangifera fruit extract increased AMPK activity by 50% compared to the positive control resveratrol and metformin, which increased AMPK activity up to 100%.

In conclusion, our in vitro results demonstrate that a fruit preparation of Mangifera indica could be useful for the treatment of metabolic disturbances.

References:

[1] Zhang BB, Zhou G, Li C. AMPK: an emerging drug target for diabetes and the metabolic syndrome. Cell Metab 2009; 9: :407 – 16

[2] Nogueiras R, Habegger KM, Chaudhary N, Finan B, Banks AS, Dietrich MO, Horvath TL, Sinclair DA, Pfluger PT, Tschöp MH. Sirtuin 1 and sirtuin 3: physiological modulators of metabolism. Physiol Rev 2012; 92: 1479 – 1514

[3] Canto C, Auwerx A. PGC-1alpha, SIRT1 and AMPK, an energy sensing network that controls energy expenditure. Curr Opin Lipidol 2009; 20: 98 – 105