Planta Med 2015; 81 - PW_175
DOI: 10.1055/s-0035-1565799

Analysis of Amaryllidaceae alkaloids in Galanthus krasnovii by GC-MS

A Emir 1, C Emir 1, B Bozkurt 1, GI Kaya 1, O Eminagaoglu 2, MA Onur 1, NU Somer 1
  • 1Department of Pharmacognosy, Faculty of Pharmacy, Ege University, 35100, Izmir, Turkey
  • 2Department of Forest Engineering, Faculty of Forestry, Artvin Coruh University, 08000, Artvin, Turkey

Galanthus L. species (Amaryllidaceae) elaborate Amaryllidaceae alkaloids which were shown to possess several pharmacological and biological activities. Therefore, they have been intensively studied for their content of alkaloids [1]. Gas Chromatography-Mass Spectroscopy (GC-MS) is a valuable tool for the detection, identification and quantification of alkaloids in Amaryllidaceae plants [2, 3]. In the present study, the alkaloidal profiles of the aerial parts and bulbs of Galanthus krasnovii Khokhr., collected from north-eastern Turkey (Kafkasor, Artvin) were investigated by GC-MS. As a result of GC-MS analysis, totally 13 alkaloids were determined. Of the known alkaloids, 11-hydroxyvittatine, anhydrolycorine, and 11,12-didehydroanhydrolycorine were found to be the main alkaloids. Also, an interesting finding of our study was the identification of an indole alkaloid, 1-acetylbetacarboline in the aerial parts of Galanthus krasnovii. This alkaloid was previously reported from Galanthus rizehensis [4].

Acknowledgement: This study was financially supported by Ege University Research Fund (Project No: 2013/ECZ/018).

References:

[1] Berkov S, Codina C, Bastida J. The Genus Galanthus: A Source of Bioactive Compounds: Rao V, editor. Phytochemicals- A global Perspective of Their Role in Nutrition and Health. InTech; 2012: 235 – 254

[2] Andrade de JP, Guo Y, Font-Bardia M, calvet T, Dutilh J, Viladomat F, Codina C, Nair JJ, Zuanazzi A, Bastida J. Crinine-type alkaloids from Hippeastrum aulicum and Hippeastrum calyptratum. Phytochemistry 2014; 103: 188 – 195

[3] Cortes N, Alvarez R, Osorio EH, Alzate F, Berkov S, Osorio E. Alkaloid metabolite profiles by GC/MS and acetylcholinesterase inhibitory activity with binding-mode predictions of five Amaryllidaceae plants. J Pharmaceut Biomed 2015; 102: 222 – 228

[4] Bozkurt Sarikaya B, Kaya GI, Onur MA, Viladomat F, Codina C, Bastida J, Unver Somer N. Alkaloids from Galanthus rizehensis. Phytochem Lett 2012; 5: 367 – 370