Planta Med 2015; 81 - PW_233
DOI: 10.1055/s-0035-1565857

Atractylosides, chlorogenic acids and antioxidant capacity of raw Arabica green coffee beans (AGCB) used by regular and spontaneous dieters to supports body weight loss and slimming

G Bazylak 1, L Marczak 2, 3, I Bielinska 2, 3, A Tadeja 1
  • 1Department of Pharmaco-Bromatology & Molecular Nutrition, Faculty of Pharmacy, Collegium Medicum, Nicolaus Copernicus University, Bydgoszcz, Poland
  • 2Department of Natural Products Biochemistry, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland
  • 3ECBiG European Centre of Bioinformatics and Genomics, Poznan, Poland

Using DPPH, ABTS and Folin-Ciocalteu procedures [1] the antioxidant capacity was characterized of fresh infusions of the raw AGCB distributed in Poland and originating from Central and South America. Next, UHPLC-TOF-MS/MS analyses of all infusions and MeOH extracts of raw AGCB were made using a Waters ACQUITY UPLC TabMode system interfaced with a Bruker microOTOF-Q mass spectrometer with an ESI source operating in negative mode at 150 °C and 4.5 kV with N2 nebulisation at 1.2 bar and a dry gas flow at 6 mL/min. An Agilent Poroshell 120EC-C18 column (2.1 × 150, 2.7 µm) was used operating at 0.6 mL/min flow rate with a mobile phase consisted of a linear gradient of 0.1% formic acid in water (A) and 0.1% formic acid in AcN (B), mixed by increasing eluate B in the range 5 – 90% from 0 – 20 min and followed by a split of column effluent flow 3:2 before the ESI ion source was used. MSMS spectra were analyzed with a Bruker Compass Data Analyzer v.4. The atractyligenin derivatives, i.e. 2-O-beta-D-glucopyranosyl-carboxyatractyligenin (m/z 525) (1), 2-O-(2'-O-isovaleryl-beta-D-glucopyranosyl)-carboxyatractyligenin (m/z 608) (2) and 3'-O-beta-D-glucopyranosyl-2'-O-isovaleryl-2-beta-(2-desoxy-carboxyatractyligenin)-beta-D-glucopyranoside (m/z 771) (3), were identified in the MeOH extracts of the raw but not milled AGCB from Colombia. Only compound 1 with a high inhibitory activity of adenine nucleotide translocase (ANT) [2] was confirmed by MSMS in the infusions of this AGCB sample. This indicates that some atractylosides in raw AGCB were hydrolyzed by hydrothermal treatment [3]. Compounds 1 and 3 with, respectively, significant and non-significant ANT inhibition were recently detected in the AGCB [2], but glucoside 2 was identified here for the first time.

References:

[1] Priori RL, Wu XL, Schaich K. J Agric Food Chem 2005; 53: 4290

[2] Lang R, Fromme T, Beusch A, Wahl A. Phytochemistry 2013; 93: 124

[3] Chen L-Y, Hu A, Chang C-J Molecules 2013; 18: 2018