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DOI: 10.1055/s-0035-1566306
Effect of Tocolytics on Surfactant Secretion When Administered with Betamethasone: An in Vitro Study
Publikationsverlauf
15. Mai 2015
01. Oktober 2015
Publikationsdatum:
03. November 2015 (online)
![](https://www.thieme-connect.de/media/ajp/201514/lookinside/thumbnails/10-1055-s-0035-1566306_150173-1.jpg)
Abstract
Objective To analyze the amount of surfactant protein (SP)-B and lecithin/sphingomyelin (L/S) ratio in response to betamethasone (BMS) alone as compared with magnesium sulfate (Mg2+), indomethacin (Indo), and nifedipine (Nif) with or without BMS.
Study design NCI-H441 human lung cells were grown and distributed into eight plates. BMS and tocolytics were added and the final plates were: control, BMS only, and each tocolytic ± BMS. Cells were stained with SP-B antibodies and relative fluorescence was measured. Lipids were also extracted, identified, and examined for relative densities. The L/S ratio was calculated.
Results Nine independent measurements were obtained for each plate. The protein analysis revealed that among all eight plates, SP-B levels were highest among BMS only. There was a nonsignificant decrease in SP-B in each of the combinations of tocolytics + BMS as compared with BMS only. Compared with BMS only, L/S ratio was decreased in Mg2+ + BMS (p = 0.041), Indo + BMS (p = 0.042), and Nif + BMS (p = 0.025).
Conclusion In our in vitro human lung cell model, SP-B and L/S ratio increased in response to BMS administration alone. The addition of tocolytics to BMS resulted in no increase in L/S ratio and no changes seen in SP-B production compared with BMS alone.
This article was presented orally at the 35th Annual Meeting of the Society for Maternal–Fetal Medicine: The Pregnancy Meeting; February 02–07, 2015; San Diego, CA.
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