Cell-Free Total and Fetal DNA in First Trimester Maternal Serum and Subsequent Development of Preeclampsia

Robert M. Silver; Leslie Myatt; John C. Hauth; Kenneth J. Leveno; Alan M. Peaceman; Susan M. Ramin; Philip Samuels; George Saade; Yoram Sorokin; Rebecca G. Clifton; Uma M. Reddy

doi:10.1055/s-0035-1570383

American Journal of Perinatology, Inhaltsverzeichnis

Am J Perinatol 2017; 34(02): 191-198
DOI: 10.1055/s-0035-1570383

Original Article

Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

Cell-Free Total and Fetal DNA in First Trimester Maternal Serum and Subsequent Development of Preeclampsia

Robert M. Silver

¹Eunice Kennedy Shriver National Institute of Child Health and Human Development, Maternal-Fetal Medicine Units Network, Bethesda, Maryland

²Department of Obstetrics and Gynecology, University of Utah, Salt Lake City, Utah

,

Leslie Myatt

¹Eunice Kennedy Shriver National Institute of Child Health and Human Development, Maternal-Fetal Medicine Units Network, Bethesda, Maryland

³University of Cincinnati, Cincinnati, Ohio

,

John C. Hauth

¹Eunice Kennedy Shriver National Institute of Child Health and Human Development, Maternal-Fetal Medicine Units Network, Bethesda, Maryland

⁴University of Alabama at Birmingham, Birmingham, Alabama

,

Kenneth J. Leveno

¹Eunice Kennedy Shriver National Institute of Child Health and Human Development, Maternal-Fetal Medicine Units Network, Bethesda, Maryland

⁵University of Texas Southwestern Medical Center, Dallas, Texas

,

Alan M. Peaceman

¹Eunice Kennedy Shriver National Institute of Child Health and Human Development, Maternal-Fetal Medicine Units Network, Bethesda, Maryland

⁶Northwestern University, Chicago, Illinois

,

Susan M. Ramin

¹Eunice Kennedy Shriver National Institute of Child Health and Human Development, Maternal-Fetal Medicine Units Network, Bethesda, Maryland

⁷Children's Memorial Hermann Hospital, University of Texas Health Science Center at Houston, Houston, Texas

,

Philip Samuels

¹Eunice Kennedy Shriver National Institute of Child Health and Human Development, Maternal-Fetal Medicine Units Network, Bethesda, Maryland

⁸The Ohio State University, Columbus, Ohio

,

George Saade

¹Eunice Kennedy Shriver National Institute of Child Health and Human Development, Maternal-Fetal Medicine Units Network, Bethesda, Maryland

⁹University of Texas Medical Branch, Galveston, Texas

,

Yoram Sorokin

¹Eunice Kennedy Shriver National Institute of Child Health and Human Development, Maternal-Fetal Medicine Units Network, Bethesda, Maryland

¹⁰Wayne State University, Detroit, Michigan

,

Rebecca G. Clifton

¹Eunice Kennedy Shriver National Institute of Child Health and Human Development, Maternal-Fetal Medicine Units Network, Bethesda, Maryland

¹¹Biostatistics Center, The George Washington University, Washington, District of Columbia

,

Uma M. Reddy

¹Eunice Kennedy Shriver National Institute of Child Health and Human Development, Maternal-Fetal Medicine Units Network, Bethesda, Maryland

› Institutsangaben

Abstract

Objective The objective of this study was to assess the relationship between first trimester cell-free total and fetal DNA in maternal plasma and the subsequent development of preeclampsia.

Study Design Nested case–control study of patients enrolled in the Combined Antioxidant and Preeclampsia Prediction Studies prediction study of 175 women who did and 175 women who did not develop preeclampsia. The predictive values of cell-free total and fetal DNA and the subsequent development of preeclampsia were measured using receiver operating characteristic curves.

Results Cell-free total DNA was higher in African American (median; 25–75%; 6.15; 0.14–28.73; p = 0.02) and Hispanic (4.95; 0.20–26.82; p = 0.037) compared with white women (2.33; 0.03–13.10). Levels of cell-free total DNA were also associated with maternal body mass index (BMI) (p = 0.02). Cell-free total DNA levels were similar between women who later developed preeclampsia (3.52; 0.11–25.3) and controls (3.74; 0.12–21.14, p = 0.96).

Conclusion There is no significant difference in levels of cell-free total DNA in the first trimester in women who subsequently develop preeclampsia. Levels of cell-free total DNA in the first trimester are increased in African American and Hispanic compared with white women, and levels increase with increasing BMI.

Keywords

preeclampsia - cell-free DNA - placenta - fetal fraction

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Referenzen

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