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Journal of Pediatric Biochemistry 2015; 05(03): 088-093
DOI: 10.1055/s-0036-1571830
Original Article
Georg Thieme Verlag KG Stuttgart · New York

Lipopolysaccharides and Prematurity: Effect on the Activity of Respiratory Chain Enzymes in Human Umbilical Venous Endothelial Cells

Thomas Neiße
1   Department of Paediatrics, Hannover Medical School, Hannover, Germany
2   Department of Dermatology and Allergy, Hannover Medical School, Hannover, Germany
,
Nils Janzen
3   Screening-Labor Hannover, Hannover, Germany
4   Department of Clinical Chemistry, Hannover Medical School, Hannover, Germany
,
Anibh M. Das
1   Department of Paediatrics, Hannover Medical School, Hannover, Germany
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Publikationsverlauf

27. Oktober 2014

16. Juni 2015

Publikationsdatum:
09. Februar 2016 (online)

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Abstract

Fetal infection is associated with considerable perinatal morbidity. Dysfunction of human umbilical venous endothelial cells (HUVEC) may compromise fetal blood supply from the placenta. HUVECs were incubated with one of the key inflammatory mediators, lipopolysaccharides (LPS), and activities of respiratory chain enzymes were measured. Based on gestational age, HUVEC were divided into a mature and two premature groups from otherwise uncomplicated pregnancies (four patients each) and incubated for 1, 6, or 12 hours with LPS (100 ng/mL), respectively, incubation without LPS served as a control. We analyzed the activities of respiratory chain enzymes and citrate synthase as a mitochondrial marker spectrophotometrically. No significant differences in respiratory chain activities between the LPS-incubated cells and internal controls could be observed. However, we were able to demonstrate significant differences depending on gestational age: respiratory chain complex IV (p < 0.01) showed higher activity and complex I + III (p = 0.01) lower activity at lower gestational age. No such differences were observed for complex II + III, complex V, and citrate synthase. No effect on the activities of mitochondrial respiratory chain enzymes induced by LPS could be shown. Activity of complex I + III was decreased and activity of complex IV was increased at lower gestational age in control and LPS-incubated cells.

Keywords

lipopolysaccharide - HUVEC - preterm - energy metabolism - respiratory chain complex activity - umbilical cord cells
 

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