Download PDF
Planta Medica International Open 2016; 3(02): e27-e30
DOI: 10.1055/s-0036-1585165
Letter
Georg Thieme Verlag KG Stuttgart · New York

Synergistic Activation of the Nrf2-ARE Oxidative Stress Response Pathway by a Combination of Botanical Extracts

Stephen R. Missler
1   Amway Corporation, Amway R&D, Ada, Michigan, USA
,
Arun Rajgopal
1   Amway Corporation, Amway R&D, Ada, Michigan, USA
,
Samantha J. Roloff
1   Amway Corporation, Amway R&D, Ada, Michigan, USA
,
Jeffrey D. Scholten
1   Amway Corporation, Amway R&D, Ada, Michigan, USA
,
Charlie R. Burns
1   Amway Corporation, Amway R&D, Ada, Michigan, USA
,
Jennifer A. Patterson
1   Amway Corporation, Amway R&D, Ada, Michigan, USA
,
Donald J. Pusateri
2   Nutrilite Health Institute, Amway R&D, Buena Park, California, USA
,
Kevin W. Gellenbeck
2   Nutrilite Health Institute, Amway R&D, Buena Park, California, USA
,
Nate P. Stern
1   Amway Corporation, Amway R&D, Ada, Michigan, USA
,
R. Keith Randolph
2   Nutrilite Health Institute, Amway R&D, Buena Park, California, USA
,
Yumei Lin
3   Yumei Consulting, Huntington Beach, California, USA
,
Jennifer Chuang
2   Nutrilite Health Institute, Amway R&D, Buena Park, California, USA
,
Valentina Kazlova
2   Nutrilite Health Institute, Amway R&D, Buena Park, California, USA
,
David J. Fast
1   Amway Corporation, Amway R&D, Ada, Michigan, USA
› Author Affiliations
Further Information

Publication History

received 25 February 2016
revised 25 February 2016

accepted 07 July 2016

Publication Date:
19 August 2016 (online)

   Permissions and Reprints

Abstract

Many fruits and vegetables have been tested for their ability to scavenge free radicals. It is more likely, however, that the therapeutic effect of dietary fruits and vegetables on oxidative stress may be due to the induction of free radical defense mechanisms via activation of the Nrf2-ARE signaling pathway. In this study, we screened botanical extracts for the ability to activate the Nrf2 pathway using an antioxidant response element luciferase reporter cell line. Extracts of turmeric, padogda tree seed pod, and rosemary exhibited activity in the reporter assay, and a combination (1 : 3 : 5 w/w/w) stimulated a synergistic response greater than expected for an additive effect of the individual extracts. The results suggest a reduction in oxidative stress with botanical extracts selected for their ability to induce the Nrf2-ARE signaling pathway may be possible in healthy individuals who consume a synergistic blend of turmeric, pagoda tree seed pod, and rosemary extracts designed to upregulate the Nrf2-ARE signaling pathway.

Key words

Curcuma longa - Zingiberaceae - Rosmarinus officinalis - Lamiaceae - Styphnolobium japonicum - Fabaceae - antioxidant response element - Nrf2
 

  • References

  • 1 Lampe JW. Health effects of vegetables and fruit: assessing mechanisms of action in human experimental studies. Am J Clin Nutr 1999; 70: 475S-490S
    PubMedSearch in Google Scholar
  • 2 Pashkow FJ. Oxidative stress and inflammation in heart disease: do antioxidants have a role in treatment and/or prevention?. Int J Inflam 2011; 2011: 514623
    PubMedSearch in Google Scholar
  • 3 Lotito SB, Frei B. Consumption of flavonoid-rich foods and increased plasma antioxidant capacity in humans: cause, consequence, or epiphenomenon?. Free Radic Biol Med 2006; 41: 1727-1746
    CrossrefPubMedSearch in Google Scholar
  • 4 Cardozo LF, Pedruzzi LM, Stenvinkel P, Stockler-Pinto MB, Daleprane JB, Leite M, Mafra D. Nutritional strategies to modulate inflammation and oxidative stress pathways via activation of the master antioxidant switch Nrf2. Biochimie 2013; 95: 1525-1533
    CrossrefPubMedSearch in Google Scholar
  • 5 Bocci V, Valacchi G. Nrf2 activation as target to implement therapeutic treatments. Front Chem 2015; 3: 4
    PubMedSearch in Google Scholar
  • 6 Harman D. Aging: a theory based on free radical and radiation chemistry. J Gerontol 1956; 11: 298-300
    CrossrefPubMedSearch in Google Scholar
  • 7 Cao G, Alessio HM, Cutler RG. Oxygen-radical absorbance capacity assay for antioxidants. Free Radic Biol Med 1993; 14: 303-311
    CrossrefPubMedSearch in Google Scholar
  • 8 Sharma OP, Bhat TK. DPPH antioxidant assay revisited. Food Chem 2009; 113: 1202-1205
    CrossrefPubMedSearch in Google Scholar
  • 9 Forman HJ, Davies KJ, Ursini F. How do nutritional antioxidants really work: nucleophilic tone and para-hormesis versus free radical scavenging in vivo . Free Radic Biol Med 2014; 66: 24-35
    CrossrefPubMedSearch in Google Scholar
  • 10 Forman HJ, Ursini F. Para-hormesis: an innovative mechanism for the health protection brought by antioxidants in wine. Nutr Aging (Amst) 2014; 2: 117-124
    PubMedSearch in Google Scholar
  • 11 United States Department of Agriculture. Oxygen Radical Absorbance Capacity (ORAC) of Selected Foods, Release 2 (2010). Available at. http://www.ars.usda.gov/Services/docs.htm?docid=15866 Access date July 1, 2016
    PubMed
  • 12 Stefanson AL, Bakovic M. Dietary regulation of Keap1/Nrf2/ARE pathway: focus on plant-derived compounds and trace minerals. Nutrients 2014; 6: 3777-3801
    CrossrefPubMedSearch in Google Scholar