Measurement of troponin-T in dried blood spots and dried plasma spots: A pilot study

Yotam Almagor; Smadar Eventov-Friedman; Amiram Nir; Awad Sror; Ann Saada

doi:10.1055/s-0036-1586475

Journal of Pediatric Biochemistry, Inhaltsverzeichnis

Journal of Pediatric Biochemistry 2014; 04(03): 153-157
DOI: 10.1055/s-0036-1586475

Review Article

Georg Thieme Verlag KG Stuttgart – New York

Measurement of troponin-T in dried blood spots and dried plasma spots: A pilot study

Autoren

Yotam Almagor

^aDepartment of Neonatology, Hadassah and Hebrew University Hospital, Jerusalem, Israel
Smadar Eventov-Friedman

^aDepartment of Neonatology, Hadassah and Hebrew University Hospital, Jerusalem, Israel
Amiram Nir

^bPediatric Cardiology Unit, Hadassah and Hebrew University Hospital, Jerusalem, Israel
Awad Sror

^cCentral Clinical Laboratory, Hadassah and Hebrew University Hospital, Jerusalem, Israel
Ann Saada

^dMonique and Jacques Roboh Department of Genetic Research, and Department of Genetics and Metabolic Diseases, Hadassah and Hebrew University Hospital, Jerusalem, Israel

Verantwortlicher Herausgeber dieser Rubrik:

Abstract

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Abstract

Troponin-T (TnT) is a biomarker for cardiac insult, but its significance as a cardiac biomarker in neonates is unclear. A method for measuring TnT in dried blood spots (DBS), which are used worldwide for neonatal screening, will enable access to a very large potential database. We tested concentrations of TnT in dried blood and dried plasma and compared results to the TnT concentrations in the original fresh blood. Troponin results in dried blood were significantly changed and did not allow sufficient test resolution. In contrast, troponin concentrations in dried plasma correlated well to those of all original samples tested. Our preliminary results suggest that TnT can accurately be measured in dried plasma, but TnT concentrations in dried blood are detectable only if troponin values are significantly elevated.

Keywords

troponin - TnT - dried blood spots - dried plasma spots - Guthrie

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