Disturbance of Microbial Core Species in New-Onset Juvenile Idiopathic Arthritis

Petra Hissink Muller; Tim G.J. de Meij; Michiel Westedt; Evelien F.J. de Groot; Cornelia F. Allaart; Danielle M.C. Brinkman; Dieneke Schonenberg-Meinema; Merlijn van den Berg; Lisette W.A. van Suijlekom-Smit; Marion van Rossum; Andries E. Budding; Rebecca ten Cate

doi:10.1055/s-0037-1601340

Journal of Pediatric Infectious Diseases, Table of Contents

J Pediatr Infect Dis 2017; 12(02): 131-135
DOI: 10.1055/s-0037-1601340

Rapid Communication

Georg Thieme Verlag KG Stuttgart · New York

Disturbance of Microbial Core Species in New-Onset Juvenile Idiopathic Arthritis

Authors

Petra Hissink Muller

¹Department of Pediatric Rheumatology, Leiden University Medical Center, Leiden, The Netherlands
Tim G.J. de Meij

²Department of Pediatric Gastroenterology, VU Medical Center, Amsterdam, The Netherlands
Michiel Westedt

¹Department of Pediatric Rheumatology, Leiden University Medical Center, Leiden, The Netherlands
Evelien F.J. de Groot

²Department of Pediatric Gastroenterology, VU Medical Center, Amsterdam, The Netherlands
Cornelia F. Allaart

³Department of Rheumatology, Leiden University Medical Center, Leiden, The Netherlands
Danielle M.C. Brinkman

¹Department of Pediatric Rheumatology, Leiden University Medical Center, Leiden, The Netherlands
Dieneke Schonenberg-Meinema

⁴Department of Pediatric Rheumatology, Emma Children's Hospital/Academic Medical Center, Amsterdam, The Netherlands
Merlijn van den Berg

⁴Department of Pediatric Rheumatology, Emma Children's Hospital/Academic Medical Center, Amsterdam, The Netherlands
Lisette W.A. van Suijlekom-Smit

⁵Department of Pediatric Rheumatology, Sophia Children's Hospital Erasmus Medical Center, Rotterdam, The Netherlands
Marion van Rossum

⁶Department of Pediatric Rheumatology, Amsterdam Rheumatology and immunology Center, Amsterdam, The Netherlands
Andries E. Budding

⁷Department of Medical Microbiology, VU University Medical Center, Amsterdam, The Netherlands
Rebecca ten Cate

¹Department of Pediatric Rheumatology, Leiden University Medical Center, Leiden, The Netherlands

Abstract

Over the past decades, the intestinal microbiota has increasingly gained attention in studies addressing the pathophysiology of (pediatric) autoimmune diseases, including inflammatory joint diseases, inflammatory bowel disease (IBD), and type 1 diabetes. In this study, we have analyzed the composition of gut microbiota of newly diagnosed juvenile idiopathic arthritis (JIA) patients, prior to initiation of disease-modifying antirheumatic drugs (DMARDs). Fecal microbiota profiles of 8 JIA patients (median age: 11.1 years, 6 girls) were compared with 22 healthy age-matched controls using IS-pro, a 16S-23S interspacer (IS) region-based, eubacterial molecular detection technique. By partial least squares discriminant analysis (PLS-DA), microbiota profiles of JIA and controls could significantly be discriminated based on a limited set of species belonging to the phylum Bacteroidetes ([Fig. 2]), but not within other phyla, with a sensitivity of 88%, specificity of 73% ([Fig. 3]), and area under the curve (AUC) 0.87 (95% CI: 0.73–0.87). These discriminative species have been considered to be part of the microbial core in healthy children.

Conclusion Our findings add to the increasing notion that the gut microbiota may be involved in the pathophysiology of JIA. Species involved in the discrimination between JIA and controls are members of the microbial core in the healthy state. Expanding knowledge on JIA-specific microbial signatures and host interactions may open avenues to explore options to develop individualized, microbiota-based preventive, and therapeutic interventions in JIA.

Keywords

autoimmunity - juvenile idiopathic arthritis - intestinal microbiota - gut - IS-pro

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References

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