HPLC-DAD studies on different Turkish oaks as potential polydatin resource

Resveratrol is now gaining much attention because of its anti-aging, antioxidant, anticancer and antioxidant effects on human health. Polydatin is a natural precursor of resveratrol. When compared with resveratrol, Polydatin has a better antioxidant effect and higher bioavailability. Enzymatic, microbiologic or chemical methods are used for transforming polydatin to resveratrol. Polygonum cuspidatum, blueberry, peanut and wine have the highest level of resveratrol [1].

In our study potential Quercus spec. are investigated as a new resource for polydatin. The genus Quercus (Fagaceae) has 23 species in the Flora of Turkey, 4 of them are endemic. In traditional Turkish medicine Quercus species have been used as an antiseptic, antidiarrheal, hemostatic, wound healing, and stomachic agent as well as a remedy for poisoning from alkaloids, copper, lead and heavy metal salts. So far flavonoids, tannins, triterpenes ionon, phenols, lignans catechin derivatives have been isolated from this species [2 – 3]. In our study, methanol and water extract of 4 Quercus spec. (Quercus cerris L. var. cerris L, Quercus macranthera Fisch. and Mey. ex Hohen subsp. syspirensis (C. Koch.), Quercus aucheri Jaub. and Spac and Quercus coccifera L.) are investigated for their polydatin content for the first time and a new HPLC-DAD method has been developed for these species. Our results showed that methanol extract of the Quercus coccifera L. have the most polydatin content (16,95%) and it could be used for the polydatin resource and for yielding resveratrol.

[1] Su D, Cheng Y, Liu M, Liu D, Cui H, Zhang B, Zhou S, Yang T, Mei Q. PloS one 2013; 8: e54505

[2] Genc Y, Yuzbasioglu M, Harput ÜŞ, Kuruuzum-Uz A. FABAD J Pharm Sci 2012; 37: 17 – 22

[3] Söhretoglu D, Kuruüzüm-Uz A, Simon A, Patócs T, Dékány M. Records of Natural Products 2014; 8: 323