Planta Medica International Open 2017; 4(S 01): S1-S202
DOI: 10.1055/s-0037-1608279
Poster Session
Georg Thieme Verlag KG Stuttgart · New York

Furochromones produced by Preussia sp.: an endophytic fungus from Casearya sylvestris var. lingua (Flacouteaceae)

R Araujo Angela
1   NuBBE – Nucleus of Bioassays, Biosynthesis and Ecophysiology of Natural Products, State University of São Paulo, Institute of Chemistry, Department of Organic Chemistry, Zip Code 14800 – 900, Araraquara, São Paulo, Brazil, Araraquara, Brazil
,
A Somensi
1   NuBBE – Nucleus of Bioassays, Biosynthesis and Ecophysiology of Natural Products, State University of São Paulo, Institute of Chemistry, Department of Organic Chemistry, Zip Code 14800 – 900, Araraquara, São Paulo, Brazil, Araraquara, Brazil
,
R Biasetto Carolina
1   NuBBE – Nucleus of Bioassays, Biosynthesis and Ecophysiology of Natural Products, State University of São Paulo, Institute of Chemistry, Department of Organic Chemistry, Zip Code 14800 – 900, Araraquara, São Paulo, Brazil, Araraquara, Brazil
,
Y Ratsbone
1   NuBBE – Nucleus of Bioassays, Biosynthesis and Ecophysiology of Natural Products, State University of São Paulo, Institute of Chemistry, Department of Organic Chemistry, Zip Code 14800 – 900, Araraquara, São Paulo, Brazil, Araraquara, Brazil
,
J Cavalheiro Alberto
1   NuBBE – Nucleus of Bioassays, Biosynthesis and Ecophysiology of Natural Products, State University of São Paulo, Institute of Chemistry, Department of Organic Chemistry, Zip Code 14800 – 900, Araraquara, São Paulo, Brazil, Araraquara, Brazil
,
LBC Camargo Ilana
2   São Carlos Institute of Physics, University of São Paulo, Zip Code 13566 – 590, São Carlos, São Paulo, Brazil, São Carlos, Brazil
,
CP Abdalla Viviani
3   Federal University of São Carlos, Botany Department, Zip Code 13565 – 905, São Carlos, São Paulo, Brazil, São Carlos, Brazil
,
S Bolzani Vanderlan
1   NuBBE – Nucleus of Bioassays, Biosynthesis and Ecophysiology of Natural Products, State University of São Paulo, Institute of Chemistry, Department of Organic Chemistry, Zip Code 14800 – 900, Araraquara, São Paulo, Brazil, Araraquara, Brazil
› Author Affiliations
Further Information

Publication History

Publication Date:
24 October 2017 (online)

 

Endophytic fungi are widespread in nature and attract considerable attention as prolific sources of new natural products with diverse biological properties and structures. Fungi of the genus Preussia sp. belong to Sporormiaceae, family (Ascomicota), have shown to be potential sources of new secondary metabolites, mainly with biological activities for drug discovery. In our continual search for bioactive compounds from endophytic fungi from Brazilian plants we have investigated Preussia sp. isolated from Casearia sylvestris var. lingua (Flacourtiaceae). Preussia sp. was cultured in corn for 28 days at 25 oC under static mode. The culture was extracted with MeOH. After evaporation of the solvent, the crude MeOH extract was partioned using water and AcOEt by liquid partitioning. The EtOAc fraction was evaporated resulting in crude extract. This extract was dissolved in CH3CN and defatted with hexane by liquid partitioning. Following, the CH3CN fraction was evaporated to give 1,100 g of the crude extract. The CH3CN extract was subjected to chromatographic separation by CC on a silica gel and preparative HPLC (reversed-phase) yielding the isolation of three compounds, (Sporormiellin B, C e D) including new compound, Sporormiellin D. The structure elucidation of these compounds were achieved by spectroscopic data, including HREIMS, 1D and 2D NMR (HMQC, HMBC, COSY, NOESY). The Sporormiellin B showed phytotoxicity activity and Sporormiellin D eradication of biofilm formed by the bacterium S. epidermidis ATCC 34984 and presented bioactivity.

CNPq, FAPESP, and CAPES.

[1] XIONG, H. et. al. Sporormiellin A, the first tetrahydrofuran-fused furochromone with an unprecedented tetracyclic skeleton from Sporormiella minima. Royal Society of Chemistry Advances, 4, 24295 – 24299, 2014.

[2] ZHANG, F. et al. Journal of Natural Products, 75, 230 – 237, 2012.