Protective effect of Saururus chinensis extract against an endocrine disruptor in mouse testicular germ cell line

This study was performed to evaluate the protective effect of Saururus chinensis ethanol extract (SCE) against styrene toxicity in mouse testicular germ cells. Cytotoxicity in mouse testicular germ cells was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Generation of reactive oxygen species (ROS) was determined using 2',7'-dichlorodihydrofluorescein diacetate (DCF-DA) assay. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and western blotting were performed to quantify the mRNA and protein expression levels, respectively, of stress or apoptosis-related genes. The results of the MTT assay showed that 50 µg/ml SCE did not affect cell viability. ROS generation in mouse spermatocyte cells increased by treatment with 100µM styrene, and decreased by co-treatment with SCE. SCE repressed the mRNA expression of stress-related genes, which increased by styrene treatment. In addition, SCE inhibited the apoptosis of mouse testicular germ cells by ameliorating mRNA and protein levels of apoptotic genes that were altered by styrene treatment. These results suggest that SCE may alleviate styrene toxicity in mouse testicular germ cells by reducing ROS stress and regulating genes related to styrene toxicity.