Recombinant Soluble P-Selectin Glycoprotein Ligand-Ig (rPSGL-Ig) Attenuates Infarct Size and Myeloperoxidase Activity in a Canine Model of Ischemia-Reperfusion

Kai Wang; Xiaorong Zhou; Zhongmin Zhou; Khaldoun Tarakji; Jian Xin Qin; Marta Sitges; Takahiro Shiota; Farhad Forudi; Robert G. Schaub; Anjali Kumar; Marc S. Penn; Eric J. Topol; A. Michael Lincoff

doi:10.1055/s-0037-1613168

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2002; 88(01): 149-154
DOI: 10.1055/s-0037-1613168

Review Article

Schattauer GmbH

Recombinant Soluble P-Selectin Glycoprotein Ligand-Ig (rPSGL-Ig) Attenuates Infarct Size and Myeloperoxidase Activity in a Canine Model of Ischemia-Reperfusion

Kai Wang

¹Department of Cardiovascular Medicine, The Cleveland Clinic Foundation, Cleveland, OH

,

Xiaorong Zhou

¹Department of Cardiovascular Medicine, The Cleveland Clinic Foundation, Cleveland, OH

,

Zhongmin Zhou

¹Department of Cardiovascular Medicine, The Cleveland Clinic Foundation, Cleveland, OH

,

Khaldoun Tarakji

¹Department of Cardiovascular Medicine, The Cleveland Clinic Foundation, Cleveland, OH

,

Jian Xin Qin

¹Department of Cardiovascular Medicine, The Cleveland Clinic Foundation, Cleveland, OH

,

Marta Sitges

¹Department of Cardiovascular Medicine, The Cleveland Clinic Foundation, Cleveland, OH

,

Takahiro Shiota

¹Department of Cardiovascular Medicine, The Cleveland Clinic Foundation, Cleveland, OH

,

Farhad Forudi

¹Department of Cardiovascular Medicine, The Cleveland Clinic Foundation, Cleveland, OH

,

Robert G. Schaub

¹Department of Cardiovascular Medicine, The Cleveland Clinic Foundation, Cleveland, OH

,

Anjali Kumar

¹Wyeth/Genetics Institute, Andover, MA, USA

,

Marc S. Penn

¹Department of Cardiovascular Medicine, The Cleveland Clinic Foundation, Cleveland, OH

,

Eric J. Topol

¹Department of Cardiovascular Medicine, The Cleveland Clinic Foundation, Cleveland, OH

,

A. Michael Lincoff

¹Department of Cardiovascular Medicine, The Cleveland Clinic Foundation, Cleveland, OH

› Author Affiliations

Abstract

Summary

The role of P-selectin in the process of reperfusion injury was evaluated using a recombinant soluble P-selectin glycoprotein ligand-Ig (rPSGL-Ig) in a canine coronary artery balloon occlusion model. rPSGL-Ig (1 mg/kg) or saline was given as an intravenous bolus 15 min before balloon deflation. Balloon occlusion time was 90 min followed by either 120 min or 7 days reperfusion. Infarct size was significantly reduced in the treatment group when expressed either as percentage of the area at risk or as absolute infarct size. Histological analysis showed that extensive myocardial injury and neutrophil infiltration were reduced by rPSGL-Ig. Myeloperoxidase activity (MPO) was significantly reduced in the risk area in the rPSGL-Ig group. Left ventricular ejection fraction was significantly less impaired during the first 24 h after reperfusion in the rPSGL-Ig group, although there was no difference by 7-day follow-up. Thus, administration of rPSGL-Ig decreases myocardial injury and inflammatory response for at least 7 days after reperfusion of ischemic myocardium.

Keywords

P-selectin - rPSGL-Ig - inflammatory response - reperfusion injury

Full Text

References

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