Platelet P-selectin expression: requirement for protein kinase C, but not protein tyrosine kinase or phosphoinositide 3-kinase

Danielle Libersan; Yahye Merhi

doi:10.1055/s-0037-1613403

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2003; 89(06): 1016-1023
DOI: 10.1055/s-0037-1613403

Platelets and Blood Cells

Schattauer GmbH

Platelet P-selectin expression: requirement for protein kinase C, but not protein tyrosine kinase or phosphoinositide 3-kinase

Danielle Libersan

¹Montreal Heart Institute and the University of Montreal, Montreal, Quebec, Canada

,

Yahye Merhi

¹Montreal Heart Institute and the University of Montreal, Montreal, Quebec, Canada

› Author Affiliations

Abstract

Summary

P-selectin is translocated from the α-granules to the surface of activated platelets where it participates in thrombosis and inflammation. We investigated the signaling pathways involved in thrombin-induced human platelet P-selectin expression. Assessed by flow cytometry, inhibition of protein kinase C (PKC) with chelerythrine reduced P-selectin expression by 66%, platelet/neutrophil binding, GPIIb/IIIa activation and aggregation (p<0.05). Gö 6976, an inhibitor of the conventional PKCs (α and β), did not alter P-selectin expression. However, rottlerin inhibited by 50% its expression (p<0.05), but only at doses that interfere with the novel (є, η) and atypical (ζ) PKCs. Inhibition of protein tyrosine kinase (PTK) and phosphoinositide 3-kinase (PI3-K) did not significantly affect P-selectin expression. In conclusion, thrombin-induced P-selectin expression is PKC-sensitive, but PTK and PI3-K-insensitive. The novel є and η and atypical ζ, but not the conventional α and β and the novel θ PKCs, may be involved in this process.

Keywords

Platelets - P-selectin - protein kinase C - protein tyrosine kinase - phosphoinositide 3-kinase

Full Text

References

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