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Thromb Haemost 2003; 89(06): 1043-1051
DOI: 10.1055/s-0037-1613406
Wound Healing and Inflammation/Infection
Schattauer GmbH

Intercellular adhesion molecule-1 (ICAM-1) expression is upregulated by thrombin in human monocytes and THP-1 cells in vitro and in pregnant subjects in vivo

Peter Clark
3   Department of Transfusion Medicine, Ninewells Hospital, Dundee, Scotland, UK
,
Fiona Jordan
1   University Department of Obstetrics and Gynaecology, Royal Infirmary, Glasgow, Scotland, UK
,
Charles Pearson
2   Department of Haematology, Royal Infirmary, Glasgow, Scotland, UK
,
Isobel D.Walker
2   Department of Haematology, Royal Infirmary, Glasgow, Scotland, UK
,
Naveed Sattar
1   University Department of Obstetrics and Gynaecology, Royal Infirmary, Glasgow, Scotland, UK
,
Joanne Ellison
1   University Department of Obstetrics and Gynaecology, Royal Infirmary, Glasgow, Scotland, UK
,
Ian A.Greer
1   University Department of Obstetrics and Gynaecology, Royal Infirmary, Glasgow, Scotland, UK
› InstitutsangabenFinancial support: The research was funded, in part, by a grant from The Laurence Scott Trust. The sponsors of the study had no role in the study design, data collection, analysis or writing of the report.
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Publikationsverlauf

Received 09. September 2002

Accepted after revision 24. März 2003

Publikationsdatum:
08. Dezember 2017 (online)

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Summary

Monocytes play a pivotal role in both the inflammatory and coagulation responses, which may be mediated through a variety of adhesion molecules on the cell surface, including intercellular adhesion molecule-1 (ICAM-1). Monocytes also possess thrombin receptors. In the current study, we have demonstrated that thrombin can upregulate ICAM-1 mRNA and induce ICAM-1 expression on the monocyte in vitro and that, in vivo, higher monocyte ICAM-1 expression is observed in pregnancy (which is characterised by a physiological increase in thrombin generation). In pregnant subjects, a positive correlation between monocyte ICAM-1 expression and a number of markers of vascular/thrombotic disease (including blood group, acquired activated protein C resistance and non-fasting plasma triglyceride levels) was observed. We also observed a significant relationship between monocyte ICAM-1 expression and soluble plasma ICAM-1 levels, which would be consistent with a contribution of monocytic ICAM-1 to the levels of free ICAM-1 observed in plasma during pregnancy.

Consistent with a role in fibrinogen binding, our preliminary in vivo results suggest that monocyte ICAM-1 expression may be a useful marker of the thrombotic/inflammatory response, although further work is required to assess the relationship of monocyte ICAM-1 expression in thrombotic disorders.

Keywords

Monocyte - ICAM-1 - thrombin - pregnancy
 

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