Deficiency of tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) impairs nutritionally induced obesity in mice

H. Roger Lijnen; Diego Demeulemeester; Berthe Van Hoef; Désiré Collen; Erik Maquoi

doi:10.1055/s-0037-1613439

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 2003; 89(02): 249-255
DOI: 10.1055/s-0037-1613439

Blood Coagulation, Fibrinolysis and Cellular Haemostasis

Schattauer GmbH

Deficiency of tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) impairs nutritionally induced obesity in mice

H. Roger Lijnen

¹Center for Molecular and Vascular Biology, University of Leuven, Leuven, Belgium

,

Diego Demeulemeester

¹Center for Molecular and Vascular Biology, University of Leuven, Leuven, Belgium

,

Berthe Van Hoef

¹Center for Molecular and Vascular Biology, University of Leuven, Leuven, Belgium

,

Désiré Collen

¹Center for Molecular and Vascular Biology, University of Leuven, Leuven, Belgium

,

Erik Maquoi

¹Center for Molecular and Vascular Biology, University of Leuven, Leuven, Belgium

› Institutsangaben

Abstract

Summary

Tissue inhibitor of matrix metalloproteinase-1 deficient (TIMP-1^–/–) mice and wild-type (TIMP-1^+/+) controls were kept on a standard (SFD) or a high fat diet (HFD) for 15 weeks. At the time of sacrifice, TIMP-1^–/– mice on HFD had a significantly lower body weight (29 ± 1.5 versus 41 ± 1.8 g, p <0.005), and significantly less subcutaneous (0.81 ± 0.19 versus 1.78 ± 0.21 g, p <0.05) and gonadal (0.87 ± 0.17 versus 1.85 ± 0.18 g, p <0.005) fat mass. These differences were much less pronounced for mice on SFD. On HFD but not on SFD, adipocyte diameters were significantly lower in the adipose tissue of TIMP-1^–/– mice. Plasma leptin levels in TIMP-1^–/– mice on HFD were significantly lower as compared to TIMP-1^+/– mice, and strongly correlated with adipose tissue mass for both genotypes. Staining with an endothelial cell specific lectin revealed a significantly higher blood vessel density, larger stained area and vessel size in adipose tissue of TIMP-1^–/– mice on HFD. This difference disappeared after normalization to the adipocyte number, suggesting that it does not represent a true enhancement of angiogenesis. Thus, in a murine model of nutritionally induced obesity, TIMP-1 promotes adipose tissue development.

Keywords

Tissue inhibitor of metalloproteinase-1 - TIMP-1 - adipose tissue - obesity - angiogenesis

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Referenzen

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