A critical role of lipid rafts in the organization of a key FcγRIIa-mediated signaling pathway in human platelets

Stéphane Bodin; Cécile Viala; Ashraf Ragab; Bernard Payrastre

doi:10.1055/s-0037-1613449

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 2003; 89(02): 318-330
DOI: 10.1055/s-0037-1613449

Platelets and Blood Cells

Schattauer GmbH

A critical role of lipid rafts in the organization of a key FcγRIIa-mediated signaling pathway in human platelets

Stéphane Bodin

¹Inserm, Unité 563, Centre de Physiopathologie de Toulouse Purpan, Department of Oncogenesis and Signaling in Haematopoïetic Cells, IFR30, Hôpital Purpan, Toulouse, France

,

Cécile Viala

¹Inserm, Unité 563, Centre de Physiopathologie de Toulouse Purpan, Department of Oncogenesis and Signaling in Haematopoïetic Cells, IFR30, Hôpital Purpan, Toulouse, France

,

Ashraf Ragab

¹Inserm, Unité 563, Centre de Physiopathologie de Toulouse Purpan, Department of Oncogenesis and Signaling in Haematopoïetic Cells, IFR30, Hôpital Purpan, Toulouse, France

,

Bernard Payrastre

¹Inserm, Unité 563, Centre de Physiopathologie de Toulouse Purpan, Department of Oncogenesis and Signaling in Haematopoïetic Cells, IFR30, Hôpital Purpan, Toulouse, France

› Institutsangaben

Abstract

Summary

The involvement of platelet FcγRIIa in heparin-associated thrombocytopenia (HIT) is now well established. However, the precise sequence of molecular events initiated by FcγRIIa cross-linking in platelets remains partly characterized. We investigated here the role of lipid rafts in the spatio-temporal organization of the FcγRIIa-dependent signaling events. Upon cross-linking, FcγRIIa relocated in rafts where the kinase Lyn and the adapter LAT were among the major phosphotyrosyl proteins. Upon stimulation by HIT sera, the second messenger phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P₃) accumulated in rafts in a P₂Y₁₂ adenosine diphosphate (ADP) recep- tor-dependent manner. PtdIns(3,4,5)P₃ was then essential to specifically recruit phospholipase Cγ2 (PLCγ2) to these membrane microdomains. Controlled disruption of rafts by methyl γ-cyclodextrin reversibly abolished PtdIns(3,4,5)P₃ production, PLC activation and platelet responses induced by FcγRIIa cross-linking without affecting the tyrosine phosphorylation events. This work demonstrates that platelet rafts are essential for the integration of a key signaling complex leading to the rapid production of PtdIns(3,4,5)P₃ and in turn PLCγ2 activation during HIT.

Keywords

Fc_γRIIa - platelets - rafts - phosphoinositides - P₂Y₁₂

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References
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