PDF herunterladen
Thromb Haemost 2000; 83(03): 503-508
DOI: 10.1055/s-0037-1613844
Review Article
Schattauer GmbH

Effect of Exercise Training on Oxidized LDL-Mediated Platelet Function in Rats

Jong Shyan Wang
3   From the Department of Physical Therapy, Chang Gung University, Taoyuan, Taiwan
,
Shu-Er Chow
1   Department of Physiology, Chang Gung University, Taoyuan, Taiwan
,
Jan-Kan Chen
1   Department of Physiology, Chang Gung University, Taoyuan, Taiwan
,
May-Kuen Wong
2   Department of Physical Medicine and Rehabilitation, Chang Gung Memorial Hospital, Taoyuan, Taiwan
› InstitutsangabenThe study was supported under the National Science Council Grant (NSC 89-2320-B-182-015)
Weitere Informationen

Publikationsverlauf

Received 23. August 1999

Accepted after resubmission 05. November 1999

Publikationsdatum:
14. Dezember 2017 (online)

Auch verfügbar auf
    Lizenzen und Reprints

Summary

This study investigates how exercise training affects Oxidized LDL (Ox-LDL) mediated-platelet activation. Five-week-old male Wistar rats were assigned to either control or trained groups. Trained rats were treadmill-trained for 10 weeks after familiarization. The following measurements were taken in both control and trained groups: Ox-LDLmediated platelet aggregability and [Ca2+]i, plasma and plateletderived nitric oxide (NO) metabolite (nitrite plus nitrate) levels, and antiaggregating activity of NO derived from endothelial cells. Based on those measurements, major findings in this study can be summarized as follows: 1) the trained group had a higher plasma -NO metabolite level than the control group; 2) the trained group had a lower platelet aggregability and [Ca2+]i elevation and a higher platelet derived-NO metabolite level than the control group; 3) the trained group had lower Ox-LDL-potentiated platelet aggregability and [Ca2+]i elevation and Ox-LDL-attenuated NO metabolite in platelet than the control group; 4) treating the platelet with L-arginine inhibited Ox-LDL-potentiated platelet activation in both control and trained groups; 5) Ox-LDL enhances platelet aggregation directly although impairing NO bioactivity but not guanylate cyclase activity in both control and trained groups. Results in this study demonstrate that exercise training decreases Ox-LDL-potentiated platelet activation most likely by enhancing platelet-derived NO release.

Key words

Exercise training - platelet - nitric oxide - Ox-LDL
 

  • References

  • 1 Pfaffenbarger RS, Hale WE, Hyde RT. Work activity and coronary heart mortality. N Engl J Med 1975; 292: 545-51.
    CrossrefPubMedGoogle Scholar
  • 2 Arraiz GA, Wigle DT, Mao Y. Risk assessment of physical activity and physical fitness in the Canada health survey mortalilty follow-up study. J Clin Epidemiol 1992; 45: 419-28.
    CrossrefPubMedGoogle Scholar
  • 3 Wang JS, Jen CJ, Chen HI. Effects of exercise training and deconditioning on platelet function in men. Arterioscl Thromb Vasc 1995; 15 (10) 1668-74.
    PubMedGoogle Scholar
  • 4 Wang JS, Jen CJ, Chen HI. Effects of chronic exercise and deconditioning on platelet function in women. J Appl Physiol 1997; 83 (06) 2080-5.
    CrossrefPubMedGoogle Scholar
  • 5 Witzum JL, Steinberg D. Role of oxidized low density lipoprotein in atherogenesis. J Clin Invest 1991; 88: 1785-92.
    CrossrefPubMedGoogle Scholar
  • 6 Parthasarathy S, Rankin SM. The role of oxidized LDL in atherogenesis. Prog Lipid Res 1992; 31: 127-43.
    CrossrefPubMedGoogle Scholar
  • 7 Koller E, Vukovich T, Doleschel W, Auerswald W. The influence of liproproteins on ADP-induced platelet aggregation. Atherogenase 1979; 04 (suppl IV 53-8.
    PubMedGoogle Scholar
  • 8 Hassall DG, Owen JS, Bruckdorfer KR. The aggregation of isolated human platelets in the presence of lipoproteins and prostacyclin. Biochem J 1983; 216: 43-9.
    CrossrefPubMedGoogle Scholar
  • 9 Chen LY, Mehta P, Mehta JL. Oxidized LDL decreases L-arginine uptake and nitric oxide synthase protein expression in human platelets: relevance of the effect of oxidized LDL on platelet function. Circulation 1996; 93: 1740-6.
    CrossrefPubMedGoogle Scholar
  • 10 Hogg N, Kalyanaraman B, Joseph J, Struck A, Parthasarathy S. Inhibition of low-density lipoprotein oxidation by nitric oxide. Potential role in atherogenesis. FEBS 1993; 334: 170-4.
    CrossrefPubMedGoogle Scholar
  • 11 Leitinger N, Oguogho A, Rodrigues M, Sinzinger H. The effect of NO/ EDRF and monocytes/macrophages on LDL-oxidation. J Physiol Pharmacol 1995; 46: 385-408.
    PubMedGoogle Scholar
  • 12 Bruckdorfer KR, Naseem KM, Mohammadi B, Chirico S, Ettelaie C, Gleeson A, Young T, Adam JM, Wilbourn B, Skinner VO, Goodall AH, Khan J, Gates SC, Jacobs M. The influence of oxidized lipoproteins, oxidation products and antioxidants on the release of nitric oxide from the endothelium and the response of platelets to nitric oxide. Biofactors 1997; 06 (02) 191-9.
    CrossrefPubMedGoogle Scholar
  • 13 Cooke JP, Tsao PS. Is NO an endogenous antiatherogenic molecule?. Arterioscl Thromb 1994; 14: 653-5.
    CrossrefPubMedGoogle Scholar
  • 14 Mackinnon LT, Hubinger L, Lepre F. Effects of physical activity and diet on lipoprotein(a). Med Sci Sport Exer 1997; 29 (11) 1429-36.
    CrossrefPubMedGoogle Scholar
  • 15 Shern-Brewer R, Santanam N, Wetzstein C, White-Welkley J, Parthasarathy S. Exercise and cardiovascular disease: a new perspective. Arterioscl Thromb Vas 1998; 18 (07) 1181-7.
    PubMedGoogle Scholar
  • 16 Brites FD, Evelson PA, Christiansen MG, Nicol MF, Basilico MJ, Wikinski RW, Llesuy SF. Soccer players under regular training show oxidative stress but an improved plasma antioxidant status. Clin Sci 1999; 96: 381-5.
    CrossrefPubMedGoogle Scholar
  • 17 Sessa WC, Pritchard K, Seyedi N, Wang J, Hintze TH. Chronic exercise in dogs increases coronary vascular nitric oxide production and endothelial cell nitric oxide synthase gene expression. Circ Res 1994; 74: 349-53.
    CrossrefPubMedGoogle Scholar
  • 18 Lin YS, Jan MS, Tsai TJ, Chen HI. Immunomodulatory effects of acute exercise bout in sedentary and trained rats. Med Sci Sports Exerc 1995; 27 (01) 73-8.
    PubMedGoogle Scholar
  • 19 Havel RJ, Eder HA, Bradgon JH. The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum. J Clin Invest 1955; 34: 1345-53.
    CrossrefPubMedGoogle Scholar
  • 20 Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979; 95: 351-8.
    CrossrefPubMedGoogle Scholar
  • 21 Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951; 193: 265-70.
    PubMedGoogle Scholar
  • 22 Allain CC, Poon LS, Chan CS, Richmond W, Fu PC. Enzymatic determination of total serum cholesterol. Clin Chem 1974; 20 (04) 470-5.
    CrossrefPubMedGoogle Scholar
  • 23 Jen CJ, Chen HI, Lai K, Usami S. Changes in cytosolic calcium concentrations and cell morphology in single platelets adhered to fibrinogen-coated surface under flow. Blood 1996; 87: 3775-82.
    CrossrefPubMedGoogle Scholar
  • 24 Thomas AP, Delaville F. The use of fluorescent indicators for measurements of cytosolic free calcium concentration in cell populations and single cells. In McCormark JG, Cobbold PH. eds. Cellular Calcium-A Practical Approach. New York: Oxford University Press; 1991: 1-53.
    Google Scholar
  • 25 Misko TP, Schilling RJ, Salvemini D, Moore WM, Currie MG. A fluorometric assay for the measurement of nitrite in biological samples. Anal Biochem 1993; 214 (01) 11-6.
    CrossrefPubMedGoogle Scholar
  • 26 Chen JK, Hoshi H, McClure DB, McKeehan WL. Role of lipoproteins in growth of human adult arterial endothelial and smooth muscle cell in low lipoprotein-deficient serum. J Cell Physiol 1986; 129: 207-14.
    CrossrefPubMedGoogle Scholar
  • 27 Minuz P, Lechi C, Gaino S, Bonapace S, Fontana L, Garbin U, Paluani F, Cominacini L, Zatti M, Lechi A. Oxidized LDL and reduction of the antiaggregating activity of nitric oxide derived from endothelial cells. Thromb Haemost 1995; 74: 1175-9.
    Artikel in Thieme ConnectPubMedGoogle Scholar
  • 28 Fletcher GF. The antiatherosclerotic effect of exercise and development of an exercise prescription. Cardiol Clin 1996; 14: 85-95 1996.
    CrossrefPubMedGoogle Scholar
  • 29 Ross R. The pathogenesis of atherosclerosis: a perspective for the 1990s. Nature 1993; 362: 801-9.
    CrossrefPubMedGoogle Scholar
  • 30 Fuhrman B, Brook GJ, Aviram M. Proteins derived from platelet a granules modulate the uptake of oxidized low density lipoprotein by macrophages. Biochem Biophys Acta 1992; 1127: 15-21.
    CrossrefPubMedGoogle Scholar
  • 31 Aviram M, Fuhrman B, Maor I, Brook GJ. Serotonin increase macrophage uptake of oxidized low density lipoprotein. Eur J Clin Chem Clin Biochem 1992; 30: 55-61.
    PubMedGoogle Scholar
  • 32 Busse R, Liickhoff A, Bassenge E. Endothelium-derived relaxant factor inhibits platelet activation. Naunyn-Schmiedeb Arch Pharmacol 1987; 336: 566-71.
    PubMedGoogle Scholar
  • 33 Radomski MW, Palmer RMJ, Moncada S. Characterization of L-arginine: nitric oxide pathway in human platelets. Br J Pharmacol 1990; 101: 325-8.
    CrossrefPubMedGoogle Scholar
  • 34 Freedman JE, Loscalzo J, Barnard MR, Alpert C, Keaney JF, Michelson AD. Nitric oxide release from activated platelets inhibits platelet recruitment. J Clin Invest 1997; 100: 350-6.
    CrossrefPubMedGoogle Scholar
  • 35 Chen H-I, Li H-T. Physical conditioning can modulate endothelium-dependent vasorelaxation in rabbit. Arterioscl Thromb 1993; 13: 852-6.
    CrossrefPubMedGoogle Scholar
  • 36 Brass LF, Hoxie JA, Manning DR. Signaling through G proteins and G protein-coupled receptors during platelet activation. Thromb Haemost 1993; 70: 217-23.
    Artikel in Thieme ConnectPubMedGoogle Scholar
  • 37 Liao JK, Shin WS, Lee WY, Clark SL. Oxidized low density lipoprotein decreases the expression of endothelial nitric oxide synthase. J Biol Chem 1995; 270: 319-24.
    CrossrefPubMedGoogle Scholar
  • 38 Hirata K, Miki N, Kuroda Y, Sakoda T, Kawashima S, Yokoyama M. Low concentration of oxidized low-density lipoprotein and lysophosphatidylcholine up-regulate constitutive nitric oxide synthase mRNA expression in bovine aortic endothelial cells. Circ Res 1995; 76: 958-62.
    CrossrefPubMedGoogle Scholar
  • 39 Ramasamy S, Parthasarathy S, Harrison DG. Regulation of endothelial nitric oxide synthase gene expression by oxidized linoleic acid. J Lipid Res 1998; 39: 268-76.
    PubMedGoogle Scholar
  • 40 Galle J, Mulsch A, Busse R, Bassenge R. Effects of native and oxidized low density lipoproteins on formation and inactivation of endothelium-derived relaxing factor. Arterioscl Thromb 1991; 11: 198-203.
    CrossrefPubMedGoogle Scholar
  • 41 Liao JK. Inhibition of G(i) proteins by low density lipoprotein attenuates bradykinin-stimulated release of endothelial-derived nitric oxide. J Biol Chem 1994; 269: 12987-92.
    PubMedGoogle Scholar
  • 42 Diaz MN, Frei B, Vita JA, Keaney JF. Antioxidants and atherosclerotic heart disease. New Engl J Med 1997; 337: 408-16.
    CrossrefPubMedGoogle Scholar