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Thromb Haemost 2000; 83(04): 545-548
DOI: 10.1055/s-0037-1613860
Commentary
Schattauer GmbH

Regional Variation in Plasminogen Activator Inhibitor-1 Expression in Adipose Tissue from Obese Individuals

Per Eriksson
1   From the Atherosclerosis Research Unit, King Gustaf V Research Institute, Karolinska Institute, Stockholm
,
Vanessa Van Harmelen
2   Department of Medicine, Huddinge Hospital, Karolinska Institute, Stockholm
,
Johan Hoffstedt
2   Department of Medicine, Huddinge Hospital, Karolinska Institute, Stockholm
,
Per Lundquist
3   Department of Surgery, Huddinge Hospital, Karolinska Institute, Stockholm, Sweden
,
Hubert Vidal
4   INSERM Unit 449, Facultéde Médecine R. Laennec, Lyon, France
,
Veronika Stemme
5   Center for Molecular Medicine, Karolinska Institute, Stockholm, Sweden
,
Anders Hamsten
1   From the Atherosclerosis Research Unit, King Gustaf V Research Institute, Karolinska Institute, Stockholm
,
Peter Arner
2   Department of Medicine, Huddinge Hospital, Karolinska Institute, Stockholm
,
Signy Reynisdottir
2   Department of Medicine, Huddinge Hospital, Karolinska Institute, Stockholm
› InstitutsangabenThis project was supported by grants from the Swedish Medical Research Council (8691, 9390, 6816 and 01034), the Swedish Heart-Lung Foundation, Swedish Diabetes Association, Novo Nordic Foundation, the European Commission (HIFMECH study, contract BMH4-CT96-0272 and FATLINK study, contract FAIR-CT-984141), the Marianne and Marcus Wallenberg Foundation, the King Gustaf V 80th Birthday Foundation, the Professor Nanna Svartz Foundation and the Swedish Society of Medicine. P. Eriksson and S. Reynisdottir are supported by post-doctoral fellowships from the Swedish Medical Research Council (12247) and Swedish National Network for Cardiovascular Research (NNCR), respectively.
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Publikationsverlauf

Received 21. Oktober 1999

Accepted 01. Dezember 1999

Publikationsdatum:
08. Dezember 2017 (online)

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Summary

High plasma plasminogen activator inhibitor-1 (PAI-1) activity is a frequent finding in obesity and adipose tissue has recently been suggested to be a source of circulating PAI-1 in humans. In the present study, differences in adipose tissue gene expression and protein secretion rate of PAI-1 between subcutaneous and visceral adipose tissue was analysed in specimens obtained from 22 obese individuals. The secretion rate of PAI-1 was two-fold higher in subcutaneous adipose tissue than in visceral adipose tissue (292 ± 50 vs 138 ± 24 ng PAI-1/107 cells, P <0.05). In accordance with the secretion data, subcutaneous adipose tissue contained about three-fold higher levels of PAI-1 mRNA than visceral adipose tissue (2.43 ± 0.37 vs 0.81 ± 0.12 attomole PAI-1 mRNA/µg total RNA, P <0.001). PAI-1 secretion from subcutaneous but not from visceral adipose tissue correlated significantly with cell size (r = 0.43, P <0.05). In summary, subcutaneous adipose tissue secreted greater amounts of PAI-1 and had a higher PAI-1 gene expression than visceral adipose tissue from the same obese individuals. Bearing in mind that subcutaneous adipose tissue is the largest fat depot these finding may be important for the coagulation abnormalities associated with obesity.

Keywords

PAI-1 - subcutaneous - visceral - obesity - TNFα
 

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