Intimal Tissue Factor Activity Is Released from the Arterial Wall after Injury

Peter L. A. Giesen; Billie S. Fyfe; John T. Fallon; Merce Roque; Milton Mendlowitz; Maria Rossikhina; Arabinda Guha; Juan J. Badimon; Yale Nemerson; Mark B. Taubman

doi:10.1055/s-0037-1613874

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 2000; 83(04): 622-628
DOI: 10.1055/s-0037-1613874

Commentary

Schattauer GmbH

Intimal Tissue Factor Activity Is Released from the Arterial Wall after Injury

Peter L. A. Giesen^*

¹Division of Thrombosis Research, New York, NY, USA

³Department of Medicine, New York, NY, USA

,

Billie S. Fyfe^**

⁴Department of Pathology, The Mount Sinai School of Medicine, New York, NY, USA

,

John T. Fallon

²The Zena and Michael A. Wiener Cardiovascular Institute, New York, NY, USA

³Department of Medicine, New York, NY, USA

⁴Department of Pathology, The Mount Sinai School of Medicine, New York, NY, USA

,

Merce Roque

²The Zena and Michael A. Wiener Cardiovascular Institute, New York, NY, USA

³Department of Medicine, New York, NY, USA

,

Milton Mendlowitz

³Department of Medicine, New York, NY, USA

,

Maria Rossikhina

²The Zena and Michael A. Wiener Cardiovascular Institute, New York, NY, USA

³Department of Medicine, New York, NY, USA

,

Arabinda Guha

¹Division of Thrombosis Research, New York, NY, USA

³Department of Medicine, New York, NY, USA

,

Juan J. Badimon

²The Zena and Michael A. Wiener Cardiovascular Institute, New York, NY, USA

³Department of Medicine, New York, NY, USA

,

Yale Nemerson

¹Division of Thrombosis Research, New York, NY, USA

³Department of Medicine, New York, NY, USA

,

Mark B. Taubman

²The Zena and Michael A. Wiener Cardiovascular Institute, New York, NY, USA

³Department of Medicine, New York, NY, USA

› Institutsangaben

Abstract

Summary

Tissue factor (TF), the initiator of coagulation, has been implicated as a critical mediator of arterial thrombosis. Previous studies have demonstrated that TF is rapidly induced in the normal rodent arterial wall by balloon injury, but is not associated with fibrin deposition. A second injury, however, performed 10–14 days after the first, is followed by small platelet-fibrin microthrombi. This study was undertaken to better localize active TF in balloon-injured rat arteries and to explore possible mechanisms underlying the apparent discrepancy between injury-induced TF expression and the lack of large platelet-fibrin thrombi. By immunohistochemistry, TF antigen was first detected in the media 24 h after injury to rat aortas, and subsequently accumulated in the neointima. Using an ex vivo flow chamber, no TF activity (Factor Xa generation) was found on the luminal surface of normal or injured aortas. Wiping the luminal surface with a cotton swab exposed TF activity in all vessels; levels were increased ≈3-fold in arteries containing a neointima. The exposed TF activity was rapidly washed into the perfusate, rendering the luminal surface inactive. The loss of luminal TF into the circulation may attenuate thrombosis at sites of arterial injury.

Keywords

Vascular smooth muscle - thrombosis - arterial injury - tissue factor

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Referenzen

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