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Thromb Haemost 2000; 83(04): 622-628
DOI: 10.1055/s-0037-1613874
Commentary
Schattauer GmbH

Intimal Tissue Factor Activity Is Released from the Arterial Wall after Injury

Peter L. A. Giesen   *
1   Division of Thrombosis Research, New York, NY, USA
3   Department of Medicine, New York, NY, USA
,
Billie S. Fyfe   **
4   Department of Pathology, The Mount Sinai School of Medicine, New York, NY, USA
,
John T. Fallon
2   The Zena and Michael A. Wiener Cardiovascular Institute, New York, NY, USA
3   Department of Medicine, New York, NY, USA
4   Department of Pathology, The Mount Sinai School of Medicine, New York, NY, USA
,
Merce Roque
2   The Zena and Michael A. Wiener Cardiovascular Institute, New York, NY, USA
3   Department of Medicine, New York, NY, USA
,
Milton Mendlowitz
3   Department of Medicine, New York, NY, USA
,
Maria Rossikhina
2   The Zena and Michael A. Wiener Cardiovascular Institute, New York, NY, USA
3   Department of Medicine, New York, NY, USA
,
Arabinda Guha
1   Division of Thrombosis Research, New York, NY, USA
3   Department of Medicine, New York, NY, USA
,
Juan J. Badimon
2   The Zena and Michael A. Wiener Cardiovascular Institute, New York, NY, USA
3   Department of Medicine, New York, NY, USA
,
Yale Nemerson
1   Division of Thrombosis Research, New York, NY, USA
3   Department of Medicine, New York, NY, USA
,
Mark B. Taubman
2   The Zena and Michael A. Wiener Cardiovascular Institute, New York, NY, USA
3   Department of Medicine, New York, NY, USA
› Author AffiliationsThis research was supported in part by National Institutes of Health Grants HL54469 and HL29019. The authors wish to thank Veronica Gulle and Norman Katz for technical assistance.
Further Information

Publication History

Received 23 August 1999

Accepted after revision 29 November 1999

Publication Date:
08 December 2017 (online)

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Summary

Tissue factor (TF), the initiator of coagulation, has been implicated as a critical mediator of arterial thrombosis. Previous studies have demonstrated that TF is rapidly induced in the normal rodent arterial wall by balloon injury, but is not associated with fibrin deposition. A second injury, however, performed 10–14 days after the first, is followed by small platelet-fibrin microthrombi. This study was undertaken to better localize active TF in balloon-injured rat arteries and to explore possible mechanisms underlying the apparent discrepancy between injury-induced TF expression and the lack of large platelet-fibrin thrombi. By immunohistochemistry, TF antigen was first detected in the media 24 h after injury to rat aortas, and subsequently accumulated in the neointima. Using an ex vivo flow chamber, no TF activity (Factor Xa generation) was found on the luminal surface of normal or injured aortas. Wiping the luminal surface with a cotton swab exposed TF activity in all vessels; levels were increased ≈3-fold in arteries containing a neointima. The exposed TF activity was rapidly washed into the perfusate, rendering the luminal surface inactive. The loss of luminal TF into the circulation may attenuate thrombosis at sites of arterial injury.

Keywords

Vascular smooth muscle - thrombosis - arterial injury - tissue factor

* Current address: Dr. P. L. A. Giesen, Synapse BV, University of Maastricht, P.O. Box 616, 6200 MD Maastricht, The Netherlands


** Current address: Dr. B. S. Fyfe, Department of Pathology and Laboratory Medicine, Medical College of Pennsylvania and Hahnemann University, Philadelphia, PA, USA


 

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