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Thromb Haemost 2000; 84(02): 325-331
DOI: 10.1055/s-0037-1614015
Review Article
Schattauer GmbH

Endothelial Cell Seeding on Crosslinked Collagen: Effects of Crosslinking on Endothelial Cell Proliferation and Functional Parameters

M. J. B. Wissink
1   From the Institute for Biomedical Technology, Polymer Chemistry and Biomaterials Group, Department of Chemical Technology, University of Twente, Enschede, The Netherlands
,
M. J. A. van Luyn
2   Faculty for Medical Sciences, Cell Biology and Biomaterials, Groningen University, Groningen, The Netherlands
,
R. Beernink
1   From the Institute for Biomedical Technology, Polymer Chemistry and Biomaterials Group, Department of Chemical Technology, University of Twente, Enschede, The Netherlands
,
F. Dijk
3   Cell Biology and Electron Microscopy, Groningen University, Groningen, The Netherlands
,
A. A. Poot
1   From the Institute for Biomedical Technology, Polymer Chemistry and Biomaterials Group, Department of Chemical Technology, University of Twente, Enschede, The Netherlands
,
G. H. M. Engbers
1   From the Institute for Biomedical Technology, Polymer Chemistry and Biomaterials Group, Department of Chemical Technology, University of Twente, Enschede, The Netherlands
,
T. Beugeling
1   From the Institute for Biomedical Technology, Polymer Chemistry and Biomaterials Group, Department of Chemical Technology, University of Twente, Enschede, The Netherlands
,
W. G. van Aken
1   From the Institute for Biomedical Technology, Polymer Chemistry and Biomaterials Group, Department of Chemical Technology, University of Twente, Enschede, The Netherlands
,
J. Feijen
1   From the Institute for Biomedical Technology, Polymer Chemistry and Biomaterials Group, Department of Chemical Technology, University of Twente, Enschede, The Netherlands
› InstitutsangabenThis study was supported by a grant of the Dutch Technology Foundation (STW).
Weitere Informationen

Publikationsverlauf

Received 03. August 1999

Accepted after resubmission 21. Februar 2000

Publikationsdatum:
14. Dezember 2017 (online)

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Summary

Endothelial cell seeding, a promising method to improve the performance of small-diameter vascular grafts, requires a suitable substrate, such as crosslinked collagen. Commonly used crosslinking agents such as glutaraldehyde and formaldehyde cause, however, cytotoxic reactions and thereby hamper endothelialization of currently available collagen-coated vascular graft materials.

The aim of this study was to investigate the effects of an alternative method for crosslinking of collagen, using N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide (EDC) in combination with N-hydroxysuccinimide (NHS), on various cellular functions of human umbilical vein endothelial cells (HUVECs) in vitro. Compared to non-crosslinked type I collagen, proliferation of seeded endothelial cells was significantly increased on EDC/NHS-crosslinked collagen. Furthermore, higher cell numbers were found with increasing crosslink densities. Neither the morphology of the cells nor the secretion of prostacyclin (PGI2), von Willebrand factor (vWF), tissue plasminogen activator (t-PA) and plasminogen activator inhibitor (PAI-1) was affected by the crosslink density of the collagen substrate. Therefore, EDC/NHScrosslinked collagen is candidate substrate for in vivo application such as endothelial cell seeding of collagen-coated vascular grafts.

Key words

Vascular grafts - collagen crosslinking - endothelial cell seeding - endothelial cell function
 

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