Quantification of Platelet-associated IgG for Differential Diagnosis of Patients with Thrombocytopenia

H. Hagenström; P. Schlenke; H. Hennig; H. Kirchner; H. Klüter

doi:10.1055/s-0037-1614115

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 2000; 84(05): 779-783
DOI: 10.1055/s-0037-1614115

Review Article

Schattauer GmbH

Quantification of Platelet-associated IgG for Differential Diagnosis of Patients with Thrombocytopenia

H. Hagenström

¹From the Institute of Immunology and Transfusion Medicine, University of Lübeck School of Medicine, Germany

,

P. Schlenke

¹From the Institute of Immunology and Transfusion Medicine, University of Lübeck School of Medicine, Germany

,

H. Hennig

¹From the Institute of Immunology and Transfusion Medicine, University of Lübeck School of Medicine, Germany

,

H. Kirchner

¹From the Institute of Immunology and Transfusion Medicine, University of Lübeck School of Medicine, Germany

,

H. Klüter

²Institute of Transfusion Medicine and Clinical Immunology, Red Cross Transfusion Service of Baden-Württemberg, Mannheim, Germany

› Institutsangaben

Abstract

Summary

Immune thrombocytopenia is due to platelet destruction by circulating glycoprotein-specific antibodies and is found in various disorders. Methods for the detection of platelet-associated IgG (PAIgG) are generally sensitive but unspecific, whereas glycoprotein-specific assays are highly specific but less sensitive. Usefully, a sensitive screening method for PAIgG detection would also provide information for differential diagnosis. We developed a quantitative direct Platelet Immunofluorescence Test (PIFT) by flow cytometry and studied 79 thrombocytopenic patients with immune thrombocytopenia and other disorders. The sensitivity of the assay was 94%, its specificity 66% for the detection of a clinically obvious immune thrombocytopenia. PAIgG levels of patients with immune thrombocytopenia differed significantly from those of other patients with low platelet counts (p <0.001). The quantitative PIFT proved to be a sensitive method for PAIgG detection and should therefore be used as a screening method. In addition, it could be helpful for differential diagnosis in marked thrombocytopenia where a MAIPA is not feasible.

Key words

Thrombocytopenia - platelet-associated IgG - flow cytometry

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Referenzen

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