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DOI: 10.1055/s-0037-1614115
Quantification of Platelet-associated IgG for Differential Diagnosis of Patients with Thrombocytopenia
We gratefully acknowledge the technical assistance of Bettina Schmitt and Michael Paulsen and thank Ms. Una Doherty for editing the manuscript. We are indebted to Prof. S. Panzer, Vienna, for proof-reading the manuscript.Publikationsverlauf
Received
24. Dezember 1999
Accepted after resubmission
30. Mai 2000
Publikationsdatum:
13. Dezember 2017 (online)
![](https://www.thieme-connect.de/media/10.1055-s-00035024/200005/lookinside/thumbnails/10-1055-s-0037-1614115-1.jpg)
Summary
Immune thrombocytopenia is due to platelet destruction by circulating glycoprotein-specific antibodies and is found in various disorders. Methods for the detection of platelet-associated IgG (PAIgG) are generally sensitive but unspecific, whereas glycoprotein-specific assays are highly specific but less sensitive. Usefully, a sensitive screening method for PAIgG detection would also provide information for differential diagnosis. We developed a quantitative direct Platelet Immunofluorescence Test (PIFT) by flow cytometry and studied 79 thrombocytopenic patients with immune thrombocytopenia and other disorders. The sensitivity of the assay was 94%, its specificity 66% for the detection of a clinically obvious immune thrombocytopenia. PAIgG levels of patients with immune thrombocytopenia differed significantly from those of other patients with low platelet counts (p <0.001). The quantitative PIFT proved to be a sensitive method for PAIgG detection and should therefore be used as a screening method. In addition, it could be helpful for differential diagnosis in marked thrombocytopenia where a MAIPA is not feasible.
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