The European Concerted Action on Anticoagulation (ECAA) Evaluation of a Set of Lyophilized Normal Plasmas to Establish the Normal Prothrombin Time for Coagulometer Systems

L. Poller; T. W. Barrowcliffe; A. M. H. P. van den Besselaar; J. Jespersen; A. Tripodi; D. Houghton; ECAA by the above steering committee; Prepared on behalf of

doi:10.1055/s-0037-1614246

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1998; 79(01): 122-128
DOI: 10.1055/s-0037-1614246

Review Article

Schattauer GmbH

The European Concerted Action on Anticoagulation (ECAA) Evaluation of a Set of Lyophilized Normal Plasmas to Establish the Normal Prothrombin Time for Coagulometer Systems

L. Poller

¹From the Central Facility, ECAA, Department of Pathological Sciences, The University of Manchester, UK

,

T. W. Barrowcliffe

²From the Division of Haematology, National Institute for Biological Standards and Control, UK

,

A. M. H. P. van den Besselaar

³From the Haemostasis and Thrombosis Research Centre, Leiden University Hospital, The Netherlands

,

J. Jespersen

⁴From the Department of Clinical Biochemistry, Centralsygehuset, Esbjerg, Denmark

,

A. Tripodi

⁵From the A. Bianchi Bonomi, Hemophilia and Thrombosis Centre, University & IRCCS Maggiore Hospital, Milan, Italy

,

D. Houghton

¹From the Central Facility, ECAA, Department of Pathological Sciences, The University of Manchester, UK

,

ECAA by the above steering committee

› Author Affiliations

Abstract

Summary

Establishing the mean normal prothrombin time (MNPT) from fresh samples for prothrombin ratios and INR often presents difficulty in selection and collection of donors. A set of seven lyophilized normal plasmas has therefore been prepared at the ECAA Central Facility and studied at 143 laboratories in sixteen European states using coagulometers in serial field exercises. All centres tested either the high ISI ECAA rabbit or low ISI ECAA human reference thromboplastin. The MNPT of fresh plasmas and means of the lyophilized samples were closely comparable with most routine rabbit thromboplastins. Using human thromboplastins means with the lyophilized normals were marginally but significantly longer and with the bovine Thrombotest significantly shorter than the MNPT of fresh plasmas causing alterations in INR. There was no appreciable effect on INR of 2.5 and 3.5 when lyophilized normals were substituted for fresh normals with the rabbit reagents.

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References

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