Nucleotide Structure and Characterization of the Murine Gene Encoding the Endothelial Cell Protein C Receptor

Zhong Liang; Elliot D. Rosen; Francis J. Castellino

doi:10.1055/s-0037-1614529

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1999; 81(04): 585-588
DOI: 10.1055/s-0037-1614529

Rapid Communication

Schattauer GmbH

Nucleotide Structure and Characterization of the Murine Gene Encoding the Endothelial Cell Protein C Receptor

Zhong Liang

¹From the Department of Chemistry and Biochemistry and the W. M. Keck Center for Transgene Research, University of Notre Dame, Notre Dame, Indiana, USA

,

Elliot D. Rosen

¹From the Department of Chemistry and Biochemistry and the W. M. Keck Center for Transgene Research, University of Notre Dame, Notre Dame, Indiana, USA

,

Francis J. Castellino

¹From the Department of Chemistry and Biochemistry and the W. M. Keck Center for Transgene Research, University of Notre Dame, Notre Dame, Indiana, USA

› Author Affiliations

Abstract

Summary

The nucleotide sequence of the entire gene encoding the murine endothelial cell receptor for activated protein C (EPCR) has been determined. A total of 5303 bp of DNA was sequenced that included 4 exons and three introns, which constituted the coding region of the gene, as well as 393 bp upstream of the first exon and 841 bp downstream of the last exon. From the locations of the introns in this gene and analysis of the exon structures, it is clear the EPCR gene is a member of the CD1 class of multiple histocompatibility proteins, and its cDNA sequence is nearly identical to that of CCD41, a centrosome-associated protein. All elements needed for RNA polymerase II-based transcription are predicted to exist in the 5’ uncoded region of the gene, and potential 3’ regulatory sequences for efficient polyadenylation have been located at their optimal locations. A variety of highly probable transcription factor binding sites have been located in the 5’ region of the gene. These data suggest that the EPCR gene is under efficient transcriptional control, and support the finding that this gene product may be involved in the inflammatory pathway.

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References

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