Expression of Tissue Factor and Tissue Factor Pathway Inhibitor in situ in Laryngeal Carcinoma

Marek Z. Wojtukiewicz; Leo R. Zacharski; Monika Ruciñska; Lech Zimnoch; Jacek Jaromin; Malgorzata Rózañska-Kudelska; Walter Kisiel; Bohdan J. Kudryk

doi:10.1055/s-0037-1614896

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 1999; 82(06): 1659-1662
DOI: 10.1055/s-0037-1614896

Rapid Communication

Schattauer GmbH

Expression of Tissue Factor and Tissue Factor Pathway Inhibitor in situ in Laryngeal Carcinoma

Marek Z. Wojtukiewicz

¹From the Departments of Oncology, Medical Academy, Bialystok, Poland

,

Leo R. Zacharski

²Department of Medicine, Dartmouth Medical School, Hanover, New Hampshire and the Department of Veterans Affairs Medical and Regional Office Center, White River Junction, Vermont, USA

,

Monika Ruciñska

¹From the Departments of Oncology, Medical Academy, Bialystok, Poland

,

Lech Zimnoch

³Pathological Anatomy, Medical Academy, Bialystok, Poland

,

Jacek Jaromin

⁴Hematology and Medical Academy, Bialystok, Poland

,

Malgorzata Rózañska-Kudelska

⁵Otolaryngology, Medical Academy, Bialystok, Poland

,

Walter Kisiel

⁶Department of Pathology, University of New Mexico, School of Medicine, Albuquerque, New Mexico, USA

,

Bohdan J. Kudryk

⁷Plasma Protein-Coagulation Laboratory, New York Blood Center, New York, USA

› Institutsangaben

Abstract

Summary

The blood coagulation mechanism may support tumor progression by several mechanisms including promotion of cell proliferation and angiogenesis. Immunohistochemical procedures were applied to AMeX-fixed sections of twelve cases of squamous cell carcinoma of the larynx obtained at surgical resection to determine the presence and distribution of tissue factor (TF), tissue factor pathway inhibitor (TFPI), other coagulation factors, fibrinogen, and fibrin in situ. TF antigen was present in normal squamous epithelial cells and tumor cells, predominantly in immature tumor cells in the vicinity of the host-tumor interface. Tumor cells stained also for factors VII and X. Staining for TFPI antigen was demonstrated in the connective tissue stroma adjacent to the tumor, in microvascular endothelial cells, and in normal squamous epithelial cells. Fibrinogen and factor XIIIa were distributed throughout the tumor connective tissue stroma. Fibrin (thrombin-cleaved fibrinogen) was detected at the host-tumor interface and along the margins of tumor nodules. Tumor cells in carcinoma of the larynx express a functional, TF-initiated pathway of blood coagulation. Interpretation of these findings together with the results of clinical trials of inhibitors of TF-induced coagulation activation versus effects of inhibitors of TF expression suggest novel approaches to the experimental therapy of laryngeal carcinoma.

Keywords

Laryngeal carcinoma - tissue factor - angiogenesis - blood coagulation

Volltext

Referenzen

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