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Thromb Haemost 1999; 82(06): 1718-1721
DOI: 10.1055/s-0037-1614904
Rapid Communication
Schattauer GmbH

Activation of Plasma Procarboxypeptidase U in Different Mammalian Species Points to a Conserved Pathway of Inhibition of Fibrinolysis

Katinka A. Schatteman
1   From the Department of Clinical Biochemistry, University of Antwerp, Wilrijk, Belgium
,
Filip J. Goossens
1   From the Department of Clinical Biochemistry, University of Antwerp, Wilrijk, Belgium
,
Simon S. Scharpé
1   From the Department of Clinical Biochemistry, University of Antwerp, Wilrijk, Belgium
,
Dirk F. Hendriks
1   From the Department of Clinical Biochemistry, University of Antwerp, Wilrijk, Belgium
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Publikationsverlauf

Received 31. Dezember 1998

Accepted after resubmission 27. Mai 1999

Publikationsdatum:
10. Dezember 2017 (online)

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Summary

Carboxypeptidase U (CPU, EC 3.4.17.20) is a recently described basic carboxypeptidase which circulates in plasma as the zymogen procarboxypeptidase U (proCPU). In the current study, we report on the presence of the proCPU/CPU system in different mammalian species – pig, guinea pig, dog, mouse, rabbit, rat and human. The proCPU concentration, determined as carboxypeptidase activity following thrombin-thrombomodulin activation, ranged from 255 U/l (mouse) to 5051 U/l (pig). When the CPU activity is generated during controlled in vitro coagulation by recalcifying citrated plasma, consistently lower activities were found compared to thrombin-thrombomodulin activation. These data indicate that in all species studied the mechanism for activation of proCPU is present. We demonstrate that in all species studied the addition of PTCI – a CPU inhibitor – results in a marked reduction of the lysis time. Albeit the presence of proCPU, the mechanism of activation during coagulation and the substantial reduction of the clot lysis time in the presence of PTCI point to a conserved inhibitory pathway of fibrinolysis.

Keywords

Fibrinolysis - carboxypeptidase U - coagulation - animal species
 

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