Thromb Haemost 1998; 79(03): 631-634
DOI: 10.1055/s-0037-1614958
Review Articles
Schattauer GmbH

Atrial Natriuretic Peptide Inhibits the Expression of Tissue Factor and Plasminogen Activator Inhibitor 1 Induced by Angiotensin II in Cultured Rat Aortic Endothelial Cells

Masami Yoshizumi
1   From the Second Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
,
Hajime Tsuji
1   From the Second Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
,
Hiromi Nishimura
1   From the Second Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
,
Teruhisa Kasahara
1   From the Second Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
,
Tatsuya Sugano
,
Haruchika Masuda
1   From the Second Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
,
Katsumi Nakagawa
1   From the Second Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
,
Yoshifumi Nakahara
1   From the Second Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
,
Hidetsugu Kitamura
1   From the Second Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
,
Keizou Yamada
1   From the Second Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
,
Mitsuru Yoneda
1   From the Second Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
,
Shohei Sawada
1   From the Second Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
,
Masao Nakagawa
1   From the Second Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
› Author Affiliations
Further Information

Publication History

Received 24 June 1997

Accepted after resubmission 09 November 1997

Publication Date:
07 December 2017 (online)

Summary

The pharmacological characteristics of atrial natriuretic peptide (ANP), such as natriuresis, vasodilation, or suppression of smooth muscle cell proliferation, are well investigated. However, this is the first study to report its role on blood coagulation and fibrinolysis mediated by vascular endothelial cells. In this study, the effects of ANP on the enhanced expression of tissue factor (TF) and plasminogen activator inhibitor 1 (PAI-1) by angiotensin II (Ang II) in cultured rat aortic endothelial cells (RAECs) were examined. The expressions of TF and PAI-1 mRNA were detected by northern blotting methods. The activities of TF on the surface of RAECs and PAI-1 in the culture media were measured by chromogenic assay. ANP suppressed mRNA expressions of TF and PAI-1 induced by Ang II in a concentration-dependent manner. This suppression was accompanied by the decreased activities of TF and PAI-1.

 
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