Atrial Natriuretic Peptide Inhibits the Expression of Tissue Factor and Plasminogen Activator Inhibitor 1 Induced by Angiotensin II in Cultured Rat Aortic Endothelial Cells

Masami Yoshizumi; Hajime Tsuji; Hiromi Nishimura; Teruhisa Kasahara; Tatsuya Sugano; Haruchika Masuda; Katsumi Nakagawa; Yoshifumi Nakahara; Hidetsugu Kitamura; Keizou Yamada; Mitsuru Yoneda; Shohei Sawada; Masao Nakagawa

doi:10.1055/s-0037-1614958

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1998; 79(03): 631-634
DOI: 10.1055/s-0037-1614958

Review Articles

Schattauer GmbH

Atrial Natriuretic Peptide Inhibits the Expression of Tissue Factor and Plasminogen Activator Inhibitor 1 Induced by Angiotensin II in Cultured Rat Aortic Endothelial Cells

Authors

Masami Yoshizumi

¹From the Second Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
Hajime Tsuji

¹From the Second Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
Hiromi Nishimura

¹From the Second Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
Teruhisa Kasahara

¹From the Second Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
Tatsuya Sugano
Haruchika Masuda

¹From the Second Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
Katsumi Nakagawa

¹From the Second Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
Yoshifumi Nakahara

¹From the Second Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
Hidetsugu Kitamura

¹From the Second Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
Keizou Yamada

¹From the Second Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
Mitsuru Yoneda

¹From the Second Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
Shohei Sawada

¹From the Second Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
Masao Nakagawa

¹From the Second Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan

Abstract

Summary

The pharmacological characteristics of atrial natriuretic peptide (ANP), such as natriuresis, vasodilation, or suppression of smooth muscle cell proliferation, are well investigated. However, this is the first study to report its role on blood coagulation and fibrinolysis mediated by vascular endothelial cells. In this study, the effects of ANP on the enhanced expression of tissue factor (TF) and plasminogen activator inhibitor 1 (PAI-1) by angiotensin II (Ang II) in cultured rat aortic endothelial cells (RAECs) were examined. The expressions of TF and PAI-1 mRNA were detected by northern blotting methods. The activities of TF on the surface of RAECs and PAI-1 in the culture media were measured by chromogenic assay. ANP suppressed mRNA expressions of TF and PAI-1 induced by Ang II in a concentration-dependent manner. This suppression was accompanied by the decreased activities of TF and PAI-1.

Full Text

References

References
1 Nishimura H, Tsuji H, Masuda H, Nakagawa K, Nakahara Y, Kitamura H, Kasahara T, Sugano T, Yoshizumi M, Sawada S, Nakagawa M. Angiotensin II increases plasminogen activator inhibitor-1 and tissue factor mRNA expression without changing that of tissue type plasminogen activator or tissue factor pathway inhibitor in cultured rat endothelial cells. Thromb Haemost 1997; 77: 1189-95.
2 Sprengers ED, Kluft C. Plasminogen activator inhibitors. Blood 1987; 69: 381-7.
3 Mcguire PG, Orkin RW. Isolation of rat aortic endothelial cells by primary explant techniques and their phenotypic modulation by defined substrata. Lab Invest 1987; 57: 94-105.
4 Drake TA, Morrissey JH, Edgington TS. Selective cellular expression of tissue factor in human tissues. Am Pathol 1989; 134: 1087-97.
5 Tanaka I, Misono KS, Inagami T. Atrial natriuretic factor in rat hypothalamus, atria and plasma: determination by specific radioimmunoassay. Biochem Biophys Res Commun 1984; 124: 663-8.
6 Lang R, Tholken EH, Canten D, Luft FC, Ruskoaho H, Unger T. Atrial natriuretic factor: a circulating hormone stimulated by volume loading. Nature 1985; 314: 264-6.
7 Mukoyama M, Nakao K, Hosoda K, Suga S, Saito Y, Ogawa Y, Shirakami G, Jougasaki M, Obata K, Yasue H, Kambayashi Y, Inouye K, Imura H. Brain natriuretic peptide (BNP) as a novel cardiac hormone in humans: Evidencc for an exquisite dual natriuretic peptide system, atrial natriuretic peptide and brain natriuretic peptide. J Clin Invest 1991; 87: 1402-12.
8 Maack T, Suzuki M, Almeida FA, Nussenzveig D, Scarborough RM, McEnroe GA, Lewicki JA. Physiological role of silent receptors of atrial natriuretic peptide. Science 1987; 238: 675-8.
9 Rathinavelu A, Isom GE. Differential internalization and processing of atrial natriuretic factor B and C receptors in PC 12 cells. Biochem J 1991; 276: 493-7.
10 Chinkers M, Garbers DL, Chang MS, Lowe DC, Chin H, Goeddel DV, Schulz S. A membrane form of guanylate cyclase is an atrial natriuretic peptide receptor. Nature 1989; 38: 78-83.
11 Chang MS, Lowe DG, Lewis M, Hellmiss R, Chin H, Goeddel DV. Differential activation by atrial and brain natriuretic peptides of two different receptor guanylate cyclases. Nature 1989; 341: 68-72.
12 Fuller F, Porter JG, Arfsten AE, Miller J, Schilling JW, Scarborough RM, Lewicki JA, Schenk DB. Atrial natriuretic peptide clearance receptor. J Biol Chem 1988; 263: 9395-401.
13 Koller KJ, Lowe DG, Bennett GL, Minamino N, Kangawa K, Matsuo H, Goeddel DV. Selective activation of the B natriuretic peptide receptor by C-type natriuretic peptide (CNP). Science 1991; 252: 120-3.
14 Ohyama Y, Miyamoto K, Morishita Y. Stable expression of natriuretic peptide receptors: effects of HS-142-1, a non-peptide ANP antagonist. Biochem Biophys Res Commun 1992; 189: 336-42.