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Thromb Haemost 1998; 79(02): 310-316
DOI: 10.1055/s-0037-1615006
Scientific and Standardization Committee Communication
Schattauer GmbH

Nucleotide Structure and Characterization of the Murine Gene Encoding Anticoagulant Protein C

Louise R. Jalbert
1   Department of Chemistry and Biochemistry and the Center for Transgene Research, University of Notre Dame, Notre Dame, Indiana, USA
,
Elliot D. Rosen
1   Department of Chemistry and Biochemistry and the Center for Transgene Research, University of Notre Dame, Notre Dame, Indiana, USA
,
Ann Lissens
2   Center for Transgene Technology and Gene Therapy, Flanders Interuniversity Institute for Biotechnology, KU Leuven, Belgium
,
Peter Carmeliet
2   Center for Transgene Technology and Gene Therapy, Flanders Interuniversity Institute for Biotechnology, KU Leuven, Belgium
,
Désiré Collen
2   Center for Transgene Technology and Gene Therapy, Flanders Interuniversity Institute for Biotechnology, KU Leuven, Belgium
,
Francis J. Castellino
1   Department of Chemistry and Biochemistry and the Center for Transgene Research, University of Notre Dame, Notre Dame, Indiana, USA
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Publikationsverlauf

Received 02. Juli 1997

Accepted after revision 23. September 1997

Publikationsdatum:
08. Dezember 2017 (online)

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Summary

The 15,160 bp murine gene encoding anticoagulation protein C (PC) was cloned and sequenced, including 414 bp upstream of exon 1 and 80 bp downstream of the translation stop codon. Nine exons and eight introns were identified. The first exon was untranslated and contained the major transcriptional start site, the surrounding nucleotide sequence of which matched reasonably well with the consensus eukaryotic Cap element sequence. The translational initiator methio-nine residue was located in exon 2. The other introns were positioned as splices between the major domain units of the protein. The 5’ untranslated region contained two possible CCAAT sequences and GC boxes, but no TATA box was obvious within the optimal range of distances from the transcription start site. The 3’-flanking nucleotides included a probable polyadenylation site (ATTAAA), beginning 80 nucleotides downstream of the translation stop codon, and a downstream consensus sequence (AGTGTTTC) required for the efficient formation of a 3’ terminus of mRNA. Several high probability transcription factor recognition sequences, including proteins that are enriched in, or specific to, the liver, such as C/EBP, C/EBP, HNF1, and HNF3, have been located in the 5’ region of the gene. These results indicate that all elements are present for liver-based transcription of the gene for murine PC.

 

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