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Thromb Haemost 1998; 79(05): 885-896
DOI: 10.1055/s-0037-1615088
Review Article
Schattauer GmbH

European Working Group on Clinical Cell Analysis: Consensus Protocol for the Flow Cytometric Characterisation of Platelet Function

Gerd Schmitz
1   From the Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Germany
,
Gregor Rothe
1   From the Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Germany
,
Andreas Ruf
1   Institute for Medical Laboratory Diagnostics, Klinikum Karlsruhe, Germany
,
Stefan Barlage
1   From the Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Germany
,
Diethelm Tschöpe
2   Diabetes Research Institute, Heinrich-Heine-University, Düsseldorf, Germany
,
Kenneth J. Clemetson
3   Theodor-Kocher-Institute, University of Bern, Switzerland
,
Alison H. Goodall
4   Department of Chemical Pathology, University of Leicester, Glenfield Hospital, United Kingdom
,
Alan D. Michelson
5   Center for Platelet Function Studies, University of Massachusetts Medical Center, Worcester, MA, USA
,
Alan T. Nurden
6   UMR 5533 CNRS, Hôpital Cardiologique, Pessac, France
,
Vincent T. Shankey
7   Urology Research, Loyola University Medical Center, Maywood, IL, USA
,
for the European Working Group on Clinical Cell Analysis › Author AffiliationsFurther Members of the Task Force Group on Platelet Immunophenotyping and Function Assays: Ault K. A., Bath P. M. W., Caldini A., Chatelain B., Gallo L., Gawaz M. P., Gratama J. W., Hjemdahl P., Holme P. A., Kappel-mayer J., Kekomäki R., Lenkei R., Martinez M. , Matic G. B., Merlin S., Neumüller J., Nieuwenhuis H. K., O’Connor J. E., Orfao A., Rapi S., Reverter J. C., Rossi E., Sansonetty F., Scharf R. E., Solum N. O., Strühn A., Valet G., Villarrubia J., Zupanska B.
Further Information

Publication History

Received 17 July 1997

Accepted after resubmission 26 January 1998

Publication Date:
07 December 2017 (online)

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Introduction

An increased or disturbed activation and aggregation of platelets plays a major role in the pathophysiology of thrombosis and haemostasis and is related to cardiovascular disease processes. In addition to qualitative disturbances of platelet function, changes in thrombopoiesis or an increased elimination of platelets, (e. g., in autoimmune thrombocytopenia), are also of major clinical relevance. Flow cytometry is increasingly used for the specific characterisation of phenotypic alterations of platelets which are related to cellular activation, haemostatic function and to maturation of precursor cells. These new techniques also allow the study of the in vitro response of platelets to stimuli and the modification thereof under platelet-targeted therapy as well as the characterisation of platelet-specific antibodies. In this protocol, specific flow cytometric techniques for platelet analysis are recommended based on a description of the current state of flow cytometric methodology. These recommendations are an attempt to promote the use of these new techniques which are at present broadly evaluated for diagnostic purposes. Furthermore, the definition of the still open questions primarily related to the technical details of the method should help to promote the multi-center evaluation of procedures with the goal to finally develop standardized operation procedures as the basis of interlaboratory reproducibility when applied to diagnostic testing.

 

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