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Thromb Haemost 1998; 80(01): 58-64
DOI: 10.1055/s-0037-1615139
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Platelet Activation and Cytokine Production during Hypothermic Cardiopulmonary Bypass – A Possible Correlation?

P. Ferroni
1   Department of Experimental Medicine and Pathology
,
G. Speziale
2   Institute of Cardiac Surgery, University of Rome La Sapienza, Rome, Italy
,
G. Ruvolo
2   Institute of Cardiac Surgery, University of Rome La Sapienza, Rome, Italy
,
A. Giovannelli
2   Institute of Cardiac Surgery, University of Rome La Sapienza, Rome, Italy
,
F. M. Pulcinelli
1   Department of Experimental Medicine and Pathology
,
L. Lenti
1   Department of Experimental Medicine and Pathology
,
P. Pignatelli
1   Department of Experimental Medicine and Pathology
,
A. Criniti
2   Institute of Cardiac Surgery, University of Rome La Sapienza, Rome, Italy
,
E. Tonelli
2   Institute of Cardiac Surgery, University of Rome La Sapienza, Rome, Italy
,
B. Marino
2   Institute of Cardiac Surgery, University of Rome La Sapienza, Rome, Italy
,
P. P. Gazzaniga
1   Department of Experimental Medicine and Pathology
› Author Affiliations
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Publication History

Received 03 June 1997

Accepted after resubmission 09 March 1998

Publication Date:
08 December 2017 (online)

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Summary

Cardiopulmonary bypass (CPB) is associated with impaired platelet function and a systemic inflammatory response. The present study was designed to evaluate whether any correlation between platelet activation and inflammatory response during CPB exists. The results obtained from 8 patients undergoing hypothermic CPB for cardiac surgery showed the occurrence of a moderate degree of platelet activation during CPB, demonstrated by an increase of platelet CD62P expression in correlation with an increase of β-thromboglobulin levels, with a concomitant decrease of in vitro platelet response. Plasma IL-1β levels significantly increased during CPB, with a peak between 1 and 4 h after CPB. Similarly, IL-6 levels were elevated 30 min from CPB starting, peaked at 4 h, and remained elevated after 24 h. A direct correlation was found between plasma IL-1β and IL-6 levels. A significant correlation between plasma IL-1β and β-thromboglobulin levels was also found. In turn, plasma β-thromboglobulin levels correlated with CD62P expression on activated platelets. An inverse correlation was found between in vitro platelet aggregation and plasma IL-1β or IL-6 levels. From the present results it may be speculated that platelet activation during CPB may contribute, through the release of IL-1β, to activation of endothelial cells and subsequent release of other cytokines with chemotactic and pro-inflammatory properties, thus playing an important role in the inflammatory response associated with CPB.

 

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