Cloning and Characterization of a cDNA Encoding Murine Coagulation Factor X

Zhong Liang; Adrian Cooper; Melanie E. DeFord; Peter Carmeliet; Desire Collen; Francis J. Castellino; Elliot D. Rosen

doi:10.1055/s-0037-1615144

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1998; 80(01): 87-91
DOI: 10.1055/s-0037-1615144

Rapid Communication

Schattauer GmbH

Cloning and Characterization of a cDNA Encoding Murine Coagulation Factor X

Zhong Liang

¹Department of Chemistry and Biochemistry and the Center for Transgene Research, University of Notre Dame, Notre Dame, Indiana, USA

,

Adrian Cooper

¹Department of Chemistry and Biochemistry and the Center for Transgene Research, University of Notre Dame, Notre Dame, Indiana, USA

,

Melanie E. DeFord

¹Department of Chemistry and Biochemistry and the Center for Transgene Research, University of Notre Dame, Notre Dame, Indiana, USA

,

Peter Carmeliet

²Center for Transgene Technology and Gene Therapy, Flanders Interuniversity Institute for Biotechnology, KU Leuven, Belgium

,

Desire Collen

²Center for Transgene Technology and Gene Therapy, Flanders Interuniversity Institute for Biotechnology, KU Leuven, Belgium

,

Francis J. Castellino

¹Department of Chemistry and Biochemistry and the Center for Transgene Research, University of Notre Dame, Notre Dame, Indiana, USA

,

Elliot D. Rosen

¹Department of Chemistry and Biochemistry and the Center for Transgene Research, University of Notre Dame, Notre Dame, Indiana, USA

› Author Affiliations

Abstract

Summary

The cDNA encoding murine coagulation factor X (fX) was isolated and reconstructed from a λZap cDNA library generated from murine liver mRNA. The cDNA contains 1486 bases starting at the 5’-translation initiation codon. It includes an open reading frame of 1443 nucleotides, followed by an 18 residue 3’ nontranslated sequence downstream of the first stop codon, and a 3’ poly(A) tail. The translation product is composed of a 40-amino acid signal/propeptide region followed by a 441-residue mature protein. The latter is highly homologous to that of human and rat fX. All protein domains of human and rat fX are strictly conserved in mouse fX. The cDNA coding for mouse fX has been expressed in human embryonic kidney 293 cells and generates fX activity measured in a clotting assay using human fX-deficient plasma.

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References

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