Evidence for the Expression of Urokinase-type Plasminogen Activator by Human Venous Endothelial Cells In Vivo

L. Camoin; R. Pannell; F. Anfosso; J. P. Lefevre; J. Sampol; V. Gurewich; F. Dignat-George

doi:10.1055/s-0037-1615396

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 1998; 80(06): 961-967
DOI: 10.1055/s-0037-1615396

Letters to the Editor

Schattauer GmbH

Evidence for the Expression of Urokinase-type Plasminogen Activator by Human Venous Endothelial Cells In Vivo

Autor*innen

L. Camoin

¹Laboratoire d’lmmuno-Hématologie, Faculté de Pharmacie
R. Pannell

³Vascular Research Laboratory, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA
F. Anfosso

¹Laboratoire d’lmmuno-Hématologie, Faculté de Pharmacie
J. P. Lefevre

²Service d’Hématologie d’Hôpital de la Conception, Marseille, France
J. Sampol

¹Laboratoire d’lmmuno-Hématologie, Faculté de Pharmacie
V. Gurewich

³Vascular Research Laboratory, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA
F. Dignat-George

¹Laboratoire d’lmmuno-Hématologie, Faculté de Pharmacie

Abstract

Summary

Endothelial cells (ECs) in culture synthesize and secrete urokinase-type plasminogen activator (u-PA), but the normal vascular endothelium is believed to synthesize only tissue plasminogen activator (t-PA), which is thought to be responsible for intravascular fibrinolysis. More recently, animal studies have shown that the biological role of u-PA in fibrinolysis has been underestimated, prompting a re-examination of its synthesis by the endothelium. In this study, we investigated whether u-PA was synthesized by non-atherosclerotic endothelial cells in vivo by testing ECs dislodged by venipuncture from 12 normal volunteers and 17 patients admitted for plasmapheresis. The ECs were isolated with an anti-endothelial monoclonal antibody coupled to immunomagnetic beads and characterized by morphology and by labelling for vWF, CD31, and UEA-1 binding. U-PA antigen was found in 50% of the ECs from the normal subjects and in 60% of those from patients. U-PA enzymatic activity on zymograms was detected in 50% of the normal samples and 60% of the patient samples, with the latter being more frequently and more strongly positive. U-PA mRNA was found in all the normal and patient samples tested. The results indicate that u-PA is synthesized by the venous endothelium in vivo but that its expression is highly variable.

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References
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