Subscribe to RSS
DOI: 10.1055/s-0037-1615440
Vesiculation and Changes in Fluidity and Lipid Composition of Platelet Membranes after Storage of Sheep Platelets in Plasma or Seto Solution
Publication History
Received
26 December 1997
Accepted after resubmission
17 June 1998
Publication Date:
08 December 2017 (online)
![](https://www.thieme-connect.de/media/10.1055-s-00035024/199804/lookinside/thumbnails/10-1055-s-0037-1615440-1.jpg)
Summary
To relate the improvement of platelet storage in synthetic media with possible structural changes, we conducted serial studies on the membranes of platelets and microparticles shed during platelet storage for up to 5 days at 4° C either in plasma or in Seto solution. Spontaneous microparticle formation proceeded linearly for up to 2 days in both storage media, although the processes seemed to be different because microparticles from Seto solution had a higher lipid/protein ratio than those released in plasma. Microparticles were heterogeneous structures showing β-N-acetylhexosaminidase, glucose-6-phosphatase and succi-nate dehydrogenase activities. After 2-5 days of storage, microparticles contained 60% of total cellular acetylcholinesterase (AChE), were doubly enriched in cholesterol, and showed identical phospholipid profiles but with a decrease in the lipid unsaturation index with respect to fresh platelets. Fluorescence anisotropy studies pointed to a remarkable increase in the deep lipid core fluidity of microparticles during storage of platelets in plasma. With respect to platelets, only those stored in plasma showed significant changes in lipid contents, with a 3-fold decrease in the phospholipid to protein ratio, a decrease in phosphatidylethanolamine (PE) levels and a parallel increase in phosphatidylcholine (PC) percentages in their phospholipid profile, together with a significant reduction in the lipid unsaturation index after 1 day of storage. The fluidity of the negatively charged surface of the platelet membranes decreased in platelets stored for 5 days in both media, whereas the fluidity of the membrane deep core was only increased in platelets stored in plasma. These findings suggest that Seto solution permits better storage of platelets for 5 days than plasma and support the notion that lipid peroxidation could play an important role in the structural changes observed.
-
References
- 1 Stack G, Snyder L. Storage of platelet concentrate. In: Blood Separation and Plasma Fractionation. Harris JR, ed. Wiley-Liss, Inc; 1991. p 99-125.
- 2 Currie LM, Harper JR, Allan H, Connor J. Inhibition of cytokine accumulation and bacterial growth during storage of platelet concentrates at 4° C with retention of in vitro functional activity. Transfusion 1997; 37: 18-24.
- 3 Adams GA, Senson SD, Ruck G. Survival and recovery of human platelets stored for five days in non-plasma medium. Blood 1986; 67: 672-5.
- 4 Shimizu T, Shibata K, Kora S. First autoclave-sterilized platelet additive solution containing glucose with a physiological pH for the preparation of plasma-poor platelet concentrates. Vox Sang 1992; 62: 87-93.
- 5 Seghatchian J, Krailadsiri P. The platelet storage lesion. Transfus Med Rev 1997; 11: 130-44.
- 6 Bode AP, Orton SM, Frye MJ, Udis BJ. Vesiculation of platelets during in vitro aging. Blood 1991; 77: 887-95.
- 7 Koerner TAW, Cunningham MT, Zhang DS. The role of membrane lipid in the platelet storage lesion. Blood Cells 1992; 18: 481-97.
- 8 Greenwalt TJ, Bryan DJ, Dumaswala UJ. Erythrocyte membrane vesiculation and changes in membrane composition during storage in citrate-phosphate-dextrose-adenine-1. Vox Sang 1984; 47: 261-70.
- 9 Dumaswala UJ, Dumaswala RU, Levin DS, Greenwalt TJ. Improved red blood cell preservation correlates with decreased loss of bands 3, 4.1, acetylcholinesterase, and lipids in microvesicles. Blood 1996; 87: 1612-6.
- 10 Fernández A, Sánchez-Yagüe J, Martín-Valmaseda EM, Felipe A, Cabezas JA, Llanillo M. Changes in the fatty acid composition of stored erythrocytes from sheep of different ages. Mech Ageing Dev 1993; 71: 189-98.
- 11 Llanillo M, Sánchez-Yagüe J, Checa A, Martín-Valmaseda EM, Felipe A. Phospholipid and fatty acid composition in stored sheep erythrocytes of different densities. Exp Hematol 1995; 23: 258-64.
- 12 Gaffet P, Bassé F, Bienvenüe A. Loss of phospholipid asymmetry in human platelet plasma membrane after 1-12 days of storage. An ESR study. Eur J Biochem 1994; 222: 1033-40.
- 13 Divers SG, Kannan K, Stewart RM, Betzing KW, Dempsey D, Fukuda M, Chervenak R, Holcombe RF. Quantitation of CD62, soluble CD62, and lysosome-associated membrane proteins 1 and 2 for evaluation of the quality of stored platelet concentrates. Transfusion 1995; 35: 292-7.
- 14 Böck M, Gawaz MP, Dietzler A, Heim MU, Mempel W. Single-donor platelet concentrates: changes of surface glycoproteins during storage. Haemostasis 1994; 24: 230-5.
- 15 Sánchez-Yagüe J, Cabezas JA, Llanillo M. Subcellular distribution and characterization of acetylcholinesterase activities from sheep platelets: relationship between temperature-dependence and environment. Blood 1990; 76: 737-44.
- 16 Wrobleski F, La Due JS. Lactate dehydrogenase activities in blood. Proc Soc Exp Biol Med 1955; 90: 210-5.
- 17 Martín-Valmaseda EM, Sánchez-Yagüe J, Cabezas JA, Llanillo M. Bio chemical characterization of sheep platelet acetylcholinesterase after detergent solubilization. Comp Biochem Physiol 1995; 110B: 91-101.
- 18 Llanillo M, Cabezas JA. Isolation, characterization and chemical composition of the membrane from sheep platelets. Biochim Biophys Acta 1981; 649: 229-38.
- 19 Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72: 248-54.
- 20 Rose HG, Oklander M. Improved procedure for the extraction of lipids from human erythrocytes. J Lipid Res 1965; 6: 428-31.
- 21 Broekhuyse RM. Quantitative two-dimensional thin-layer chromatography of blood phospholipids. Clin Chim Acta 1969; 23: 457-61.
- 22 Sánchez-Yagüe J, Llanillo M. Lipid composition of subcellular particles from sheep platelets. Location of phosphatidylethanolamine and phosphatidylserine in plasma membranes and platelet liposomes. Biochim Biophys Acta 1986; 856: 193-201.
- 23 Jain SK. Evidence for membrane lipid peroxidation during the in vivo aging of human erythrocytes. Biochim Biophys Acta 1988; 937: 205-10.
- 24 Shinitzky M, Barenholz Y. Fluidity parameters of lipid regions determined by fluorescence polarization. Biochim Biophys Acta 1978; 515: 367-94.
- 25 George JN, Pickett EB, Heinz R. Platelet membrane microparticles in blood bank fresh frozen plasma and cryoprecipitate. Blood 1986; 68: 307-9.
- 26 Comfurius P, Senden JMG, Tilly RHJ, Schroit AJ, Bevers EM, Zwaal RFA. Loss of membrane phospholipid asymmetry in platelets and red cells may be associated with calcium-induced shedding of plasma membrane and inhibition of aminophospholipid translocase. Biochim Biophys Acta 1990; 1026: 153-60.
- 27 Pessina GP, Ciccoli L, Fanetti G. Fatty acid composition of erythrocyte and vesicle total lipids during storage of human erythrocytes in protein-free media and in citrate-phosphate-dextrose. Ital J Biochem 1988; 37: 293-301.
- 28 Zwaal RFA, Schroit AJ. Pathophysiologic implications of membrane phospholipid asymmetry in blood cells. Blood 1997; 89: 1121-31.
- 29 Frenkel EJ, Kuyper FA, Op den Kamp JAF, Roelofsen B, Ott P. Effect of membrane cholesterol on dimyristoylphosphatidylcholine-induced vesiculation of human red blood cells. Biochim Biophys Acta 1986; 855: 293-301.
- 30 Yano Y, Kambayashi JI, Shiba E, Sakon M, Oiki E, Fukuda K, Kawasaki T, Mori T. The role of protein phosphorylation and cytoskeletal reorganization in microparticle formation from the platelet plasma membrane. Biochem J 1994; 299: 303-8.
- 31 Okuma M, Steiner M, Baldini M. Lipid content and in vitro incorporation of free fatty acids into lipids of human platelets: The effect of storage at 4° C. Blood 1971; 38: 27-38.
- 32 Hamid MA, Kunichi TJ, Aster RH. Lipid composition of freshly prepared and stored platelet concentrates. Blood 1980; 55: 124-30.
- 33 Bressler NM, Broekman MJ, Marcus AJ. Concurrent studies of oxygen consumption and aggregation in stimulated human platelets. Blood 1979; 53: 167-74.
- 34 Fagiolo E, Lippa S, Mores N, Oradei A, Aureli V. Peroxidative events in stored platelet concentrates. Vox Sang 1989; 56: 32-6.
- 35 Mateo CR, Lillo MP, González-Rodríguez J, Acuña AU. Molecular order and fluidity of the plasma membrane of human platelets from time-resolved fluorescence depolarization. Eur Biophys J 1991; 20: 41-52.
- 36 Kitagawa S, Matsubayashi M, Kotame K, Usui K, Kametani F. Asymmetry of membrane fluidity in the lipid bilayer of blood platelet: fluorescence study with diphenylhexatriene and analogs. J Membrane Biol 1991; 119: 221-7.
- 37 Marcus AJ, Ullman HL, Safier LB. Lipid composition of subcellular particles of human blood platelets. J Lipid Res 1969; 10: 108-14.
- 38 Jarolín P, Mircevová L. Changes in fluidity of erythrocyte membranes after storage of erythrocytes and regeneration of cellular ATP level. Biochim Biophys Acta 1982; 688: 460-4.
- 39 Tozzi-Ciancarelli MG, D’Alfonso A, Tozzi E, Troiani-Sevi E, De Matteis G. Fluorescence studies of the aged erythrocyte membranes. Cell Mol Biol 1989; 35: 113-20.
- 40 Schroeder F, Kier AB, Sweet DW. Role of polyunsaturated fatty acids and lipid peroxidation in LM fibroblast plasma membrane transbilayer structure. Arch Biochem Biophys 1990; 276: 55-64.
- 41 Berlin E, Shapiro SG, Friedland M. Platelet membrane fluidity and aggregation of rabbit platelets. Atherosclerosis 1984; 51: 223-39.
- 42 Caimi G, Lo Presti R, Montana M, Canino B, Ventimiglia G, Romano A, Catania A, Sarno A. Membrane fluidity, membrane lipid pattern, and cytosolic Ca2+ content in platelets from a group of type II diabetic patients with macrovascular complications. Diabetes Care 1995; 18: 60-3.
- 43 Jain SK, McVie R, Duett J, Herbst JJ. Erythrocyte membrane lipid peroxidation and glycosylated hemoglobin in diabetes. Diabetes 1989; 38: 1539-43.
- 44 Opper C, Clement C, Schwarz H, Krappe J, Steinmetz A, Schneider J, Wesemann W. Increased number of high sensitive platelets in hypercholesterolemia, cardiovascular diseases and after incubation with cholesterol. Atherosclerosis 1995; 113: 211-7.
- 45 Bassé F, Gaffet P, Bienvenüe A. Correlation between inhibition of cyto-skeleton proteolysis and antivesiculation effect of calpeptin during A23187-induced activation of human platelets: are vesicles shed by filopod fragmentation?. Biochim Biophys Acta 1994; 1190: 217-24.
- 46 Murphy S, Shimizu T, Miripol J. Platelet storage for transfusion in synthetic media: further optimization of ingredients and definition of their roles. Blood 1995; 86: 3951-60.
- 47 Tan KH, Meyer DJ, Belin J, Ketterer B. Inhibition of microsomal lipid peroxidation by gluthatione and gluthatione transferases B and AA. Role of endogenous phospholipase A2 . Biochem J 1984; 220: 243-52.