Pulmonary Fibrosis Is Increased in Mice Carrying the Factor V Leiden Mutation following Bleomycin Injury

Zuojun Xu; Randal J. Westrick; YueChun Shen; Daniel T. Eitzman

doi:10.1055/s-0037-1615623

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2001; 85(03): 441-444
DOI: 10.1055/s-0037-1615623

Scientific and Standardization Committee Communication

Schattauer GmbH

Pulmonary Fibrosis Is Increased in Mice Carrying the Factor V Leiden Mutation following Bleomycin Injury

Zuojun Xu

¹Division of Cardiology, Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, MI, USA

,

Randal J. Westrick

¹Division of Cardiology, Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, MI, USA

,

YueChun Shen

¹Division of Cardiology, Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, MI, USA

,

Daniel T. Eitzman

¹Division of Cardiology, Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, MI, USA

› Author Affiliations

Abstract

Summary

Increased fibrin deposition following inflammatory lung injury has been proposed to facilitate the development of pulmonary fibrosis. Therefore, factors predisposing to thrombosis may affect the fibrotic response to injury. Activated protein C (aPC) resistance due to the factor V Leiden mutation (FνL) is a common genetic risk factor for vascular thrombosis. To examine the relationship between aPC resistance and the development of pulmonary fibrosis, lung inflammation was induced by bleomycin in mice carrying the FvL mutation. Three weeks following the instillation of 0.0375 U of bleomycin, the lungs of mice homozygous and heterozygous for FvL contained significantly more hydroxyproline (35 ± 4 and 36 ± 7 ug hydroxyproline/ mg total protein, respectively) than wild-type mice (26 ± 6 ug/mg protein, p <0.01 for both comparisons). These data demonstrate a strong relationship between aPC resistance and the pulmonary fibrosis that occurs following inflammatory lung injury.

Keywords

Pulmonary fibrosis - thrombosis - bleomycin - factor V Leiden - fibrin

Full Text

References

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