Absence of Fibrinogen in Afibrinogenemia Results in Large but Loosely Packed Thrombi under Flow Conditions

Jasper A. Remijn; Ya-Ping Wu; Martin J. W. IJsseldijk; Jaap J. Zwaginga; Jan J. Sixma; Philip G. de Groot

doi:10.1055/s-0037-1615661

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2001; 85(04): 736-742
DOI: 10.1055/s-0037-1615661

Review Articles

Schattauer GmbH

Absence of Fibrinogen in Afibrinogenemia Results in Large but Loosely Packed Thrombi under Flow Conditions

Jasper A. Remijn

¹Thrombosis and Haemostasis Laboratory, Department of Haematology, University Medical Center, Utrecht, The Netherlands

,

Ya-Ping Wu

¹Thrombosis and Haemostasis Laboratory, Department of Haematology, University Medical Center, Utrecht, The Netherlands

,

Martin J. W. IJsseldijk

¹Thrombosis and Haemostasis Laboratory, Department of Haematology, University Medical Center, Utrecht, The Netherlands

,

Jaap J. Zwaginga

¹Thrombosis and Haemostasis Laboratory, Department of Haematology, University Medical Center, Utrecht, The Netherlands

,

Jan J. Sixma

¹Thrombosis and Haemostasis Laboratory, Department of Haematology, University Medical Center, Utrecht, The Netherlands

,

Philip G. de Groot

¹Thrombosis and Haemostasis Laboratory, Department of Haematology, University Medical Center, Utrecht, The Netherlands

› Author Affiliations

Abstract

Summary

We studied the role of fibrinogen in platelet thrombus formation under flow on adhesive proteins using afibrinogenemic blood (LMWH anticoagulated) in a perfusion system. Perfusions with afibrinogenemic blood showed strong increased surface coverage and thrombus volume that normalized upon addition of fibrinogen. Similar studies using citrate anticoagulated blood showed that this was due to fibrinogen and not fibrin. Morphological analysis showed that afibrinogenemic thrombi were loosely packed and consisted mainly of dendritic platelets that contacted one another through filopodia. However, in the presence of fibrinogen, platelets formed lamellipodia and spread out on top of one another. Studies with radiolabeled platelets showed similar numbers of platelets in both conditions demonstrating that the difference is one of packing and the larger size is due to absence of lamellipodia formation and spreading. The found increased thrombus size and loosely packed platelets might help to understand thrombotic complications sometimes seen in afibrinogenemia patients.

Keywords

Afibrinogenemia - thrombus - flow - fibrinogen - platelet

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References

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