F X Nottingham and F X Taunton Two Novel Mutations in Factor X Resulting in Loss of Functional Activity and an Interpretation Using Molecular Modelling

S. Deam; N. Srinivasan; J. Westby; E.H. Horn; G. Dolan

doi:10.1055/s-0037-1615695

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2001; 85(02): 265-269
DOI: 10.1055/s-0037-1615695

Review Article

Schattauer GmbH

F X Nottingham and F X Taunton Two Novel Mutations in Factor X Resulting in Loss of Functional Activity and an Interpretation Using Molecular Modelling

S. Deam

¹Departments of Haematology and Clinical Chemistry, Queen’s Medical Centre, Nottingham, UK

,

N. Srinivasan

²Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India

,

J. Westby

¹Departments of Haematology and Clinical Chemistry, Queen’s Medical Centre, Nottingham, UK

,

E.H. Horn

³Department of Haematology, Royal Infirmary, Edinburgh, UK

,

G. Dolan

¹Departments of Haematology and Clinical Chemistry, Queen’s Medical Centre, Nottingham, UK

› Author Affiliations

Abstract

Summary

We report two novel mutations in the Factor X gene which result in a bleeding tendency in two unrelated Caucasian families. Although the mutations occur at adjacent codons in exon 8 and result in reduced functional activity with normal antigen levels, the patterns of inheritance appear to be quite distinct. Factor X Nottingham (alanine 404 threonine) appears to be associated with an autosomal recessive pattern of inheritance. In contrast, Factor X Taunton (arginine 405 glycine) results in a mode of inheritance consistent with an autosomal dominant pattern, all five of the heterozygotes in this family being clinically affected. Molecular modelling studies suggest that, in the case of Factor X Nottingham, a drastic conformational change causes major unfolding of the protein. For Factor X Taunton, less extreme conformational changes occur causing loss of functional activity such that substrate binding sites might be maintained. It is proposed that competition with wild type for substrate binding could occur leading to a dominant negative effect.

Key words

Factor X mutations molecular modelling

Full Text

References

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