Factor XIII of Blood Coagulation as a Nuclear Crosslinking Enzyme

Róza Ádány; Helga Bárdos; Miklós Antal; László Módis; Attila Sárváry; Sándor Szücs; Imre Balogh

doi:10.1055/s-0037-1615758

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2001; 85(05): 845-851
DOI: 10.1055/s-0037-1615758

Review Article

Schattauer GmbH

Factor XIII of Blood Coagulation as a Nuclear Crosslinking Enzyme

Róza Ádány^*

¹Department of Preventive Medicine, School of Public Health

,

Helga Bárdos^*

¹Department of Preventive Medicine, School of Public Health

,

Miklós Antal

²Department of Anatomy, Histology and Embryology, Medical and Health Science Center, University of Debrecen, Hungary

,

László Módis

²Department of Anatomy, Histology and Embryology, Medical and Health Science Center, University of Debrecen, Hungary

,

Attila Sárváry

¹Department of Preventive Medicine, School of Public Health

,

Sándor Szücs

¹Department of Preventive Medicine, School of Public Health

,

Imre Balogh

¹Department of Preventive Medicine, School of Public Health

› Author Affiliations

Abstract

Summary

Intracellular localization and distribution of Factor XIII subunit A (FXIIIA) was investigated in association with monocyte-macrophage differentiation in a long term culture of human monocytes by light-and electron microscopical as well as biochemical and immunobio-chemical techniques. To allow the detection of FXIIIA in cells with well-preserved ultrustructure, immunosera against glutaraldehydederivatized recombinant FXIIIA were developed in rabbits, then characterized and used in this study. In the early phase of macrophage differentiation intranuclear accumulation of FXIIIA was detected as a transient phenomenon in cells of the 2nd day culture by optical sectioning with 0,7 m steps in laser scanning confocal microscopy and immunoblotting technique. FXIIIA could be detected by immuno-electron microscopic postembedding staining over electrodense DNA-containing areas. Fluoresceinated monodansylcadaverine incorporation assay was used to demonstrate that FXIIIA is not only present in the nuclei, but also expresses its transglutaminase activity. Our finding of the nuclear accumulation of FXIIIA in differentiating human macrophages is also unique in that a blood clotting factor has, for the first time, been localized in nuclei and has been shown to be an intracellular crosslinking enzyme. The possible role of nuclear FXIIIA in association with cellular processes involving chromatin structure remodeling, such as cell death, cell differentiation or cellular proliferation requires further in-depth investigation.

Keywords

Factor XIII - transglutaminase - cell-nucleus - monocyte-macrophage differentiation - confocal microscopy

Full Text

References

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